Effects of pH (a) and temperature (b) on the activities of TanLpl (•), TanLpa (□), TanLpe (△), and A. oryzae tannase (×). HPLC analysis was performed under various conditions for the hydrolysis of methyl gallate. pH experiments were performed at 37°C, and temperature experiments were performed at pH 8.0 and 5.5 for lactobacilli tannase and A. oryzae tannase, respectively. The values are shown as the relative activity, and the maximum relative activities are indicated as 100%. Each experiment was performed in triplicate.