TY - JOUR AU - Ueda, Shuhei AU - Nomoto, Ryohei AU - Yoshida, Ken-ichi AU - Osawa, Ro PY - 2014 DA - 2014/04/07 TI - Comparison of three tannases cloned from closely related lactobacillus species: L. Plantarum, L. Paraplantarum, and L. Pentosus JO - BMC Microbiology SP - 87 VL - 14 IS - 1 AB - Tannase (tannin acyl hydrolase, EC 3.1.1.20) specifically catalyzes the hydrolysis of the galloyl ester bonds in hydrolyzable tannins to release gallic acid. The enzyme was found not only in fungal species but also many bacterial species including Lactobacillus plantarum, L. paraplantarum, and L. pentosus. Recently, we identified and expressed a tannase gene of L. plantarum, tanLpl, to show remarkable differences to characterized fungal tannases. However, little is known about genes responsible for tannase activities of L. paraplantarum and L. pentosus. We here identify the tannase genes (i.e. tanLpa and tanLpe) of the above lactobacilli species, and describe their molecular diversity among the strains as well as enzymological difference between species inclusive of L. plantarum. SN - 1471-2180 UR - https://doi.org/10.1186/1471-2180-14-87 DO - 10.1186/1471-2180-14-87 ID - Ueda2014 ER -