Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick

Table 3 Samples of Candidatus Liberibacter asiaticus positive material used to evaluate the Las -LAMP assay

Species	Sample name	Origin		Detection method
		Host	Country	*Las*-LAMP gel	*Las*-LAMP LFD
Candidatus Liberibacter asiaticus	CLAS-12	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CLAS-13	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CLAS-14	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CLAS-15	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CLAS-16	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CLAS-17	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CLAS-18	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CLAS-19	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CLAS-20	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CLAS-21	Orange	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-1	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-2	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-3	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-4	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-5	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-6	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-7	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-8	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-9	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-10	Psyllid	Brazil	+	+
Candidatus Liberibacter asiaticus	CHICHALAS-11	Psyllid	Brazil	+	+

For each isolate the Las-LAMP reaction was performed in triplicate. Gel: gel electrophoresis. LFD: lateral flow dipstick. +: Positive reaction. -: Negative reaction.

ISSN: 1471-2180