Figure 1

Effect of human serum on C. albicans biofilms. A) Analysis of biofilm formation in the presence of normal human serum (HS). ATCC90028 was grown in polypropylene microtiter plates at 37°C for 24 h in the presence of different concentrations of HS. a. Scanned image of the XTT reduction assay for quantitation of biofilms. b. Quantitation of biofilms by XTT reduction assay. B) Different concentrations of HS were added to pre-adhered biofilms of ATCC90028 and incubated in RPMI 1640 medium for an additional 24 h at 37°C. a. Scanned image of the XTT reduction assay for quantitation of biofilms. b. Quantitation of biofilms by XTT reduction assay. All experiments were done in triplicate with three technical repeats on separate days with similar results and shown as a representative image. RPMI 1640/HS vs. RPMI 1640, **p < 0.01.