Cytokine response in vaccinated mice following immunization and L. donovani challenge infection. Ten days post-vaccination and 4 months after L. donovani challenge infection splenocytes were restimulated in vitro with LAg (10 μg/mL) in media alone or in the presence of anti-CD4 or anti-CD8 monoclonal antibody (1 μg/106 cells). After 72 h supernatants were collected and assayed for IL-12 ((A, B), IFN-γ (C, D), IL-4 (E, F) and IL-10 (G, H)) by ELISA. Each sample was examined in duplicate. The results are shown as the mean ± SE for five individual mice per group, representative of two independent experiments with similar results. * p < 0.05, ** p < 0.01, *** p < 0.001 in comparison to PBS as well as free adjuvant immunized groups as assessed by one-way ANOVA and Tukey’s multiple comparison test.