Figure 3

DTH responses in vaccinated mice following immunization and L. donovani challenge infection. LAg-specific DTH responses were measured ten days post-vaccination, or 2 and 4 months after challenge infection. DTH response is expressed as the difference (in millimeters) between the thickness of the test (LAg-injected) and control (PBS-injected) footpads at 24 h. Bars represent the mean ± SE of five individual mice per group, and are representative of two independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 in comparison to PBS as well as free adjuvant immunized groups as assessed by one-way ANOVA and Tukey’s multiple comparison test.