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Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus

Abstract

Background

Transketolase (TKT) is a key enzyme of the pentose phosphate pathway (PPP), the Calvin cycle and the ribulose monophosphate (RuMP) cycle. Bacillus methanolicus is a facultative RuMP pathway methylotroph. B. methanolicus MGA3 harbors two genes putatively coding for TKTs; one located on the chromosome (tkt C) and one located on the natural occurring plasmid pBM19 (tkt P).

Results

Both enzymes were produced in recombinant Escherichia coli, purified and shown to share similar biochemical parameters in vitro. They were found to be active as homotetramers and require thiamine pyrophosphate for catalytic activity. The inactive apoform of the TKTs, yielded by dialysis against buffer containing 10 mM EDTA, could be reconstituted most efficiently with Mn2+ and Mg2+. Both TKTs were thermo stable at physiological temperature (up to 65°C) with the highest activity at neutral pH. Ni2+, ATP and ADP significantly inhibited activity of both TKTs. Unlike the recently characterized RuMP pathway enzymes fructose 1,6-bisphosphate aldolase (FBA) and fructose 1,6-bisphosphatase/sedoheptulose 1,7-bisphosphatase (FBPase/SBPase) from B. methanolicus MGA3, both TKTs exhibited similar kinetic parameters although they only share 76% identical amino acids. The kinetic parameters were determined for the reaction with the substrates xylulose 5-phosphate (TKTC: kcat/KM: 264 s-1 mM-1; TKTP: kcat/KM: 231 s-1 mM) and ribulose 5-phosphate (TKTC: kcat/KM: 109 s-1 mM; TKTP: kcat/KM: 84 s-1 mM) as well as for the reaction with the substrates glyceraldehyde 3-phosphate (TKTC: kcat/KM: 108 s-1 mM; TKTP: kcat/KM: 71 s-1 mM) and fructose 6-phosphate (TKTC kcat/KM: 115 s-1 mM; TKTP: kcat/KM: 448 s-1 mM).

Conclusions

Based on the kinetic parameters no major TKT of B. methanolicus could be determined. Increased expression of tkt P, but not of tkt C during growth with methanol [J Bacteriol 188:3063–3072, 2006] argues for TKTP being the major TKT relevant in the RuMP pathway. Neither TKT exhibited activity as dihydroxyacetone synthase, as found in methylotrophic yeast, or as the evolutionary related 1-deoxyxylulose-5-phosphate synthase. The biological significance of the two TKTs for B. methanolicus methylotrophy is discussed.

Background

Transketolase (TKT, EC 2.2.1.1) catalyzes the cleavage of a carbon-carbon bond adjacent to a carbonyl group in ketosugars and transfers a two-carbon ketol group to aldosugars [1, 2], a reaction that might already have occurred under prebiotic conditions [3]. TKT requires divalent cations and thiamine diphosphate (ThDP) as a cofactor for its activity [4]. TKT is a key enzyme of the non-oxidative branch of the pentose phosphate pathway (PPP), the Calvin cycle and the ribulose monophosphate (RuMP) cycle. In these metabolic pathways, two ketol group transfers are relevant, the interconversion of xylulose 5-phosphate (X5-P) and ribose 5-phosphate (R5-P) to sedoheptulose 7-phosphate (S7-P) and glyceraldehyde phosphate (GAP) and the interconversion of GAP and fructose 6-phosphate (F6-P) to erythrose 4-phosphate (E4-P) and X5-P [5]. These substrates of TKT are important as precursors e.g. for nucleotide biosynthesis (R5-P), biosynthesis of aromatic amino acids (E4-P) and for cell wall biosynthesis in Gram-negative bacteria (S7-P). They are also intermediates of central pathways of carbon metabolism e.g. glycolysis (F6-P and GAP) and the Calvin and RuMP pathways [6].

TKT occurs in animals, plants, yeasts, archaea and bacteria like Corynebacterium glutamicum[7]. Properties of purified TKT have been reported mostly for eukaryotes like baker’s yeast [4], spinach [8], rat liver [9], mouse brain [10], human leukocytes/erythrocytes [11] but also from bacteria such Escherichia coli[12]. TKT is usually a homodimer with two active centers located at the interface between the contacting monomers. Methylotrophic yeasts possess a related enzyme, dihydroxyacetone synthases (DHAS, EC 2.2.1.3), which catalyzes the two-carbon ketol transfer from X5-P to formaldehyde yielding dihydroxyacetone phosphate (DHAP) and GAP. Thus, in these yeasts formaldehyde is assimilated by DHAS and the products DHAP and GAP are further metabolized to regenerate the X5-P and in other reactions of the central carbon metabolism [13]. DHAS has been purified from Candida boidinii[13] and from the carboxydobacterium Acinetobacter sp. [14] and is likely to be present in the actinomycete Amycolatopsis methanolica[15]. Besides DHAS and TKT also DHAS-like proteins have been described, but their function remains unknown [16].

The Gram-positive, thermotolerant and facultative methylotrophic bacterium Bacillus methanolicus that can use the one-carbon (C1) compound methanol as a source of carbon and energy [17–19] possesses two genes annotated to encode TKT [20]. One of them is encoded on the chromosome (tkt C), while the other one was found on the natural occurring plasmid pBM19 (tkt P) [20, 21]. While the enzymes have not yet been characterized it has been proposed that they play an important role in the PPP and the RuMP pathway [20, 22].

The initial reaction of methanol utilization in B. methanolicus is the oxidation of methanol to formaldehyde catalyzed by methanol dehydrogenase (MDH) [18]. It is known that B. methanolicus possesses three distinct active MDHs [23]. Reduction equivalents are generated by the linear dissimilation pathway of formaldehyde to CO2 and also by the PPP [24, 25]. However, no formaldehyde dehydrogenase (FADH) was found in B. methanolicus[21]. Formaldehyde assimilation in B. methanolicus occurs via the RuMP pathway, which is divided in three different parts: fixation, cleavage and regeneration. The key reactions of the RuMP cycle are the aldol condensation of formaldehyde with ribulose 5-phosphate by 3-hexulose-6-phosphate synthase (HPS) and the subsequent isomerization of the product, D-arabino-3-hexulose 6-phosphate, to fructose 6-phosphate by 6-phospho-3-hexuloisomerase (PHI) in the fixation part. Fructose 1,6-bisphosphate (FBP) is generated in the subsequent phosphofructokinase reaction (Figure 1). Fructose 1,6-bisphosphate aldolase (FBA, EC 4.1.2.13) cleaves FBP into GAP and DHAP. B. methanolicus has one chromosomal- and one plasmid-encoded FBA (FBAP and FBAC, respectively). Both catalyze the reversible cleavage of FBP to the triose phosphates GAP and DHAP [26]. We recently showed that FBAP is presumably the major gluconeogenic FBA while FBAC is the major glycolytic FBA in this bacterium [26]. The regeneration phase of the RuMP pathway, in which Ru5-P is regenerated, shares enzymes with the PPP and glycolysis [27] (Figure 1). Two different variants of the regeneration part of the RuMP pathway are known, the TA (transaldolase) variant and the SBPase (sedoheptulose 1,7-bisphosphatase) variant. Based on its genome sequence, B. methanolicus possesses the whole genetic equipment for both variants of the RuMP pathway [20–22]. In the TA variant, S7-P is directly generated from F6-P and E4-P by a TA activity, while the SBPase variant requires the activity of a sedoheptulose 1,7-bisphosphate aldolase (SBA) to generate sedoheptulose 1,7-bisphosphate (SBP) and an SBPase activity for the further conversion of SBP to S7-P. We recently demonstrated, that both FBAs from B. methanolicus are promiscuous enzymes also active as SBA, while only the plasmid encoded GlpXP was active as FBPase and SBPase, which indicates that the SBPase variant of the RuMP pathway might operate in this organism [28]. Three enzymes, transketolase (TKT), ribose 5-phosphate isomerase (RPI) and ribulose 5-phosphate 3-epimerase (RPE), are shared in both variants. In the RuMP pathway, the predicted function of the TKT(s) is identical to the PPP and Calvin cycle.

Figure 1
figure 1

Proposed map of the biochemical reactions of the methanol oxidation and assimilation pathways in B. methanolicus including the TA (dashed arrows) and the SBPase (solid arrows) variants of the RuMP pathway. Enzymes: MDH, methanol dehydrogenase (EC 1.1.1.244); HPS, 3-hexulose-6-phosphate synthase (EC 4.1.2.43); PHI, 6-phospho-3-hexuloisomerase (EC 5.3.1.27); PFK, 6-phosphofructokinase, (EC 2.7.1.11); FBA, fructose-bisphosphate aldolase (EC 4.1.2.13); TKT, transketolase (EC 2.2.1.1); GlpX, fructose-bisphosphatase (EC 3.1.3.1); TA, transaldolase (EC 2.2.1.2); RPE, ribulose- phosphate 3-epimerase (EC 5.1.3.1); RPI, ribose-5-phosphate isomerase (EC 5.3.1.6); Metabolites: H6-P, 3-hexulose 6-phosphate; F6-P, fructose-6-phosphate; FBP, fructose-1,6-bisphosphate; GAP, glyceraldehyde 3-phosphate; DHAP, dihydroxyacetone phosphate; E4-P, erythrose 4-phosphate; SBP, sedoheptulose 1,7-bisphosphate; S7-P, sedoheptulose-7-phosphate; Ri5-P, ribose 5-phosphate; X5P, xylulose 5-phosphate; Ru5P, ribulose 5-phosphate; The reactions are described in detail in the text. Adapted from [28].

It has been shown that the natural plasmid pBM19 carries the key mdh gene and five genes with deduced roles in the RuMP pathway (glpX, fba, tkt, pfk, rpe). The absence of pBM19 results in the loss of the ability to grow on methanol and caused higher methanol tolerance and reduced formaldehyde tolerance levels in B. methanolicus cells [20]. Transcription levels of mdh and the five plasmid encoded RuMP pathway genes, as well as the chromosomal genes hps and phi, were increased during growth with methanol suggesting their importance for methylotrophy [21, 22]. Notably, 15 fold higher mRNA tkt P levels were found in methanol- as compared to mannitol-grown cells [21, 22]. Methanol consumption by this organism involves the concerted recruitment of both plasmid and chromosomal genes, and this discovery represented the first documentation of plasmid dependent methylotrophy [20, 22, 29].

The plasmid- encoded enzymes characterized to date differ from their chromosomally encoded counterparts as e.g. the three MDH enzymes exhibit different biochemical and physical properties and their genes are regulated differently [23]. GlpXC was shown to be the major FBPase of B. methanolicus, while GlpXP also carries SBPase activity [28]. Both FBAC and FBAP are SBAs, but their kinetic parameters allowed to distinguish FBAC as major glycolytic FBA and FBAP as major gluconeogenic FBA [26]. The objective of this study was to characterize the role and enzymatic properties of the two TKTs from B. methanolicus to get further insight into the genetic and biochemical aspects of methylotrophy

Results

Bioinformatic analysis and phylogeny of the TKTP and TKTC from B. methanolicus

B. methanolicus possesses two distinct genes encoding TKT [21], tkt C on the chromosome and the plasmid located tkt P. The deduced primary sequences of these proteins show a similarity of 87% (578/668) and an identity of 76% (506/668) to each other. The closest homolog of TKTC present in the database is the chromosomally encoded homolog (EIJ77615.1; 97% identical amino acids) of B. methanolicus strain PB1. Similarly, the closest homolog of plasmid encoded TKTP is the TKT (EIJ81398.1) from B. methanolicus PB1 (95% identical amino acids), which is encoded on plasmid pBM20. BLAST analyses of the amino acid sequences of TKTC and TKTP as queries suggested their classification as TKT with more than 200 sequences sharing 50% or more identical amino acids. An amino acid sequence alignment with biochemically characterized and experimentally verified TKTs from E. coli K12, encoded by tktA and tktB[12, 30, 31], Plasmodium falciparum, encoded by pftk[32], Leishmania mexicana, encoded by tkt[33], Trypanosoma brucei, encoded by tbtkt[34], and Saccharomyces cerevisiae, encoded by sctk[35] revealed the presence of highly conserved amino acid residues throughout the sequence with two notable motifs (Figure 2). The first N- terminal located motif is common to all Thiamindiphosphat (ThDP)-dependent enzymes. The sequence begins with the highly conserved residues Gly-Asp-Gly (GDG) followed by 21 less conserved residues [36, 37]. The second so-called Tk motif is specific for all TKTs [38].

Figure 2
figure 2

Primary sequence alignment of TKT proteins. Black and grey boxes indicate identical and similar residues. Bars indicate the characteristic ThDP motif and the TK motif. The sequence alignment was carried out using ClustalW, the alignment was formatted using BoxShade.

Overexpression of tkt C and tkt P resulted in increased TKT activity in B. methanolicus

In order to study if the tkt C and tkt P genes encode functionally active TKT enzymes, both genes were overexpressed in B. methanolicus. Plasmids pTH1-tkt C and pTH1-tkt P were constructed based on pTH1 and with the tkt coding sequences under control of the methanol-inducible mdh promoter [20, 39] and used to transform B. methanolicus. To confirm overexpression, TKT activities were determined in crude extracts of the resulting recombinant cells after growth in SOBSuc medium with or without 200 mM methanol. B. methanolicus carrying the empty vector pTH1 showed similar TKT activities regardless of the presence of the inducer (0.073 ± 0.004 U mg-1 under non-inducing conditions and of 0.075 ± 0.005 U mg-1 when methanol was present as inducer). When induced by methanol, the overexpression strains carrying either pTH1-tkt C or pTH1-tkt P showed significantly increased TKT activities of 0.373 ± 0.052 and 0.351 ± 0.064 U mg-1, respectively, as compared to non-inducing conditions (0.082 ± 0.002 and 0.083 ± 0.003 U mg-1, respectively). Thus, overexpression of tkt C and tkt P indeed increased transketolase activities 4–5 fold, confirming that both genes encode functionally active TKTs.

Heterologous expression, purification and biochemical characterization of the TKTP and TKTC

(I) Overexpression, purification and molecular mass detection

The tkt P and tkt C coding regions were PCR-amplified and cloned into pET16b for production of the enzymes with an N-terminal His-tag (Table 1). The resulting plasmids were transformed into E. coli BL21 (DE3) and recombinant protein production was induced by the addition of IPTG to exponentially growing cell cultures. Cells were harvested, crude extracts were prepared and after Ni-NTA chromatography, His-tags were cleaved using factor Xa, and the enzymes were buffered in 50 mM Tris–HCl (pH 7.7). Protein purifications from 500 ml of culture broth led to average concentrations of about 1.2 mg/ml for both enzymes and a total amount of about 3 mg per purification.

Table 1 List of strains and plasmids used

Gel filtration of both proteins and TKT activity assays of the eluted fractions showed that both proteins eluted in a single fraction indicating that they are active as homotetramers with molecular weights for the tetramers of 280 kDa.

(II) Determining the optimal conditions for TKT activity

The optimal assay conditions of the TKT enzymes were determined by using a coupled spectrometric assay for measuring the formation of GAP from R5-P and X5-P (as described in Materials and Methods). The activity of the auxiliary enzymes TPI and GPD were first checked under the different conditions and added in excess. Measurements were performed in 50 mM Tris–HCl buffer at 55°C and by using substrate concentrations of 1 mM for both TKTC and TKTP, which is 7 and 5 times greater than the determined KM values for TKTC and TKTP, respectively (see below) Activity could be measured for both enzymes within a broad pH range between 6.5-10 for TKTC and 5.5-9 for TKTP with a pH optimum of pH 7.2-7.4 for both enzymes. All subsequent assays were performed at pH 7.5, the putative physiologically relevant pH.

The influence of the temperature, the pH, the effect of some metal ions and effectors were analyzed using enzyme Assay I (see materials and Methods). TKT activity in different buffers was tested and found to be almost independent of the buffer substance used in concentrations between 20 mM and 200 mM. Phosphate buffer, however, showed an inhibitory effect of the TKT activity of approximately 40%.

The highest activity of both TKTs was determined around 62°C, which corresponds roughly to the upper limit growth temperature of B. methanolicus. Temperatures higher than these resulted in strongly decreased TKT activities, which could be, to some extent, explained by the instability of the substrates triose phosphates [44] and/or reflect denaturation of the enzymes.

(III) TKT C displays higher temperature stability than TKT P

The thermal stability of both TKTs was tested by pre-incubation of the proteins at temperatures ranging from 40 to 80°C. Samples were taken in different time periods and the activity was measured at 50°C under standard conditions. Both TKTs remained stable up to 50°C for at least 2 hours. Upon pre-incubation at 60°C the catalytic activity was reduced for both enzymes to approximately 60% within 10 minutes and then remained stable at this level. Incubation at 70°C led to a complete loss of activity for TKTC after 4 minutes, for TKTP after 30 minutes of incubation.

(IV) Formation of the TKT apoform and reconstitution of the holoenzyme revealed a bivalent metal ion dependency for activity

During optimization of the assay conditions for the TKT activity, a dependence of bivalent cation for both TKTs was observed. Therefore, the apo-TKT form was obtained for both B. methanolicus TKTs by removing any bound cofactors via dialysis for 24 hours against Tris–HCl buffer containing 10 mM EDTA. After EDTA was removed by subsequent dialysis, different divalent metal ions, including Co2+, Ni2+, Cu2+, Mn2+, Mg2+ and Ca2+ were tested as putative cofactors for both TKTs at a final concentration of 1 mM (Figure 3). Reconstitution of the TKT activity was stimulated by Mn2+, Mg2+, Co2+, Ca2+ and Cu2+. The addition of Ni2+ did not restore the TKT activity at all, while slow reconstitution was observed with water, presumably due to contamination of substrates or buffer components with divalent cations.

Figure 3
figure 3

Reconstitution of apoforms of TKT C (A) and TKT P (B) in the presence of different divalent cations. The reaction was measured according to the enzyme assay I (Methods) with the standard substrates R5-P and X5-P and dialyzed TKT preparations. Each reaction mixture contained 1 mM divalent cations and 150 ng purified TKT enzyme. At t = 0, the assay was started by the addition of THDP to a final concentration of 20 μM. The decrease in absorbance at 340 nm as a result of NADH oxidation was monitored over time.

(V) TKT activities are inhibited by ATP, ADP, EDTA and Ni 2+

To identify inhibitors or activators of B. methanolicus TKT activity, potential effectors were tested at concentrations of 1 and 5 mM. TKTP and TKTC were both inhibited by ATP (65% and 75%, respectively) and by ADP (65% and 95%, respectively). EDTA in concentration of 10 mM resulted for both TKT in a completely loss of activity. Ni2+ at a concentration of 1 mM also led to a complete loss of activity for both TKT.

TKTP and TKTC share similar kinetic parameters and substrate spectrum

The kinetic parameters of TKTC and TKTP were determined for the conversion of F6-P and GAP to X5-P and E4-P as well as for the formation of S7-P and GAP from X5-P and R5-P in vitro (Table 2). The assays were performed at 60°C and pH 7.5 in 50 mM Tris–HCl with 2 mM MnCl2 and 1 μM THDP. Both recombinant TKTs catalyzed the conversion of X5-P and R5-P to GAP and S7-P with comparable kinetic parameters. For X5-P and TKTC a KM of 150 μM ± 4 μM and a Vmax of 34 ± 1 U/mg could be determined, whereas TKTP displayed a KM of 232 μM ± 2 μM and Vmax of 45 ± 1 U/mg. Similar parameters could be measured for the second substrate R5-P, for which TKTC has a KM of 118 μM ± 13 μM and a Vmax of 11 ± 1 U/mg, TKTP shows a KM of 250 μM ± 13 μM and Vmax of 18 ± 1 U/mg. The catalytic efficiencies for both TKTs are accordingly quite similar for X5-P (for TKTC 264 s–1 mM–1 and for TKTP 231 s–1 mM–1) and this also holds for R5-P (for TKTC 109 s–1 mM–1 and for TKTP 84 s–1 mM–1). Comparable catalytic efficiencies could be calculated for GAP (for TKTC 108 s–1 mM–1 and for TKTP 71 s–1 mM–1) while for F6-P the catalytic efficiency for TKTP is about 4-fold higher than that of TKTC (448 s–1 mM–1 and 115 s–1 mM–1, respectively) Following affinities were observed for GAP (TKTC KM 0.92 ± .033 mM and a Vmax 85 ± 3 U/mg; TKTP KM 0.67 ± .012 mM and Vmax 42 ± 4 U/mg) and F6-P (TKTC KM 0.72 ± 0.11 mM and a Vmax of 71 ± 11 U/mg; TKTP: KM 0.25 mM and Vmax 96 ± 5 U/mg).

Table 2 Biochemical properties of TKT P and TKT C

The kinetics of the reverse reactions could not be determined since neither E4-P nor S7-P are currently available commercially. An additional activity as DHAS, as found in methylotrophic yeasts, or as the evolutionary related DXP synthase could not be observed.

Discussion

The biochemical results provided here show that the plasmid (TKTP) and chromosomally (TKTP) encoded TKTs are similar and based on these data it is not feasible to predict their individual roles for methylotrophy in B. methanolicus. Both TKTs are active as homotetramers, a characterisitic shared with TKTs from Triticum aestivum and Sus scrova[5], but different from several microbial TKTs such as the enzymes from E. coli[12, 45], Saccharomyces cerevisiae[46] and Rhodobacer sphaeroides[47]. The requirement of bivalent cations for the activity of TKT from B. methanolicus with a preference of Mn2+. Mg2+, and Ca2+ is a common feature of TKTs, while the efficiency for the cations varies between different TKTs [12, 48]. It was assumed in the past, that purified mammalian TKTs do not require the addition of cofactors to maintain activity [9]. This led to the wrong conclusion that these enzymes did not require bivalent cations for activity. This was because the complex of TKT with THDP and cation is strong enough to carry the cofactors along the purification steps and though TKT remaining active. The cation can be removed by dialysis against EDTA [9, 49, 50]. Both TKTs showed comparable biochemical properties. This is in contrast to the recently characterized and biochemically diverse MDHs from B. methanolicus, which displayed different biochemical and regulatory properties [23]. Both TKTs were shown to be thermo stable at physiological temperature (50°C) of B. methanolicus. Neutral pH (6.5 to 7.8) was also reported to be optimal for both enzymes of E. coli[13, 31] and S. cerevisiae[51] and Rhodobacter sphaeroides[47]. Inhibition by ATP and ADP is unusual, however, since the intracellular concentrations of ATP and ADP in B. methanolicus are not known, it is difficult to judge the relevance of this inhibition in vivo.

TKT has been found so far in all organisms that have been investigated [31]. The presence of more than one TKT however, as described here for B. methanolicus is not a common phenomenon. Two TKTs are known in S. cerevisiae, encoded by tkl1 and tkl2[52, 53], and E. coli, encoded by tktA and tktB[12, 30]. As in B. methanolicus, the TKTs of E. coli and S. cerevisiae exhibit comparable kinetic parameters. However, deletion of tkl1, probably encoding the main TKT in S. cerevisiae, impaired growth in synthetic medium without added aromatic amino acids, whereas deletion of tkl2 did not cause such phenotype. In E. coli, the tktA gene product is the major isoenzyme and accounts for about 70 to 90% of TKT activity in cells and tktA mutants are highly sensitive to the presence of D-ribose, while tktB deletion mutants are not. tktA tktB double mutants are viable, but deficient in pentose catabolism and they require the addition of all three aromatic amino acids, aromatic vitamins and pyridoxine (vitamin B6). Transketolase A from Escherichia coli was shown to derepress the multiple antibiotic resistance operon marRAB by binding to the repressor MarR [54]. It remains to be shown if the TKTs from B. methanolicus show regulatory interactions with transcriptional repressors and if TKTP and TKTC differ in this respect.

Besides the common sugar phosphates F6-P, R5-P, GAP, X5-P and E4-P, TKTs from spinach leaves and S. cerevisiae are able to also utilize DHAP, dihydroxyacetone (DHA) and HP [50, 55, 56]. The reaction of TKTs with formaldehyde (called DHAS) is known in methylotrophic yeasts [57] and was recently also reported for transketolase A of E. coli[31]. However, among all substrates tested, both TKTs form B. methanolicus were only active with X5-P and R5-P as well as F6-P and GAP. Similar substrate specificity was described for mammalian TKTs [58]. Based on the catalytic efficiency (TKTC 82 s–1 mM–1 versus TKTP 448 s–1 mM–1) TKTP appears better suited for the interconversion of S7-P and GAP to R5-P and X5-P.

About 15 fold higher mRNA levels of tktP, but not of tktC, were previously observed when comparing growth in minimal medium with methanol and mannitol [21]. This induction was not observed here when assaying crude extracts of B. methanolicus MGA3(pTH1) which carries endogenous plasmid pBM19 after growth in complex medium SOBSuc induced with 200 mM methanol. Likely, this difference is due to the use of different media, namely complex medium with methanol vs. methanol minimal medium.

Conclusion

Both, TKTP and TKTC, showed comparable kinetic parameters. The about 15 fold increased mRNA levels of tkt P and of other RuMP pathway genes on the plasmid pBM19, which is essential for methanol utilization [12, 21] during growth in methanol minimal medium as compared to growth in mannitol minimal medium [20] argues for TKTP being the major TKT relevant in the RuMP pathway. In line with this argumentation, methanol-inducible GlpXP carries SBPase activity, which is relevant in the RuMP pathway [28], while the chromosomally encoded GlpXC is the major FBPase in gluconeogenesis and is not methanol-inducible.

Methods

Microorganisms and cultivation conditions

B. methanolicus strains were grown at 50°C in the following media. SOBsuc medium is SOB medium (Difco) supplemented with 0.25 M sucrose. Bacterial growth was performed in shake flasks (500 ml) in 100 ml medium at 200 r.p.m. and monitored by measuring the OD600. The inoculation of the precultures for all growth experiments of B. methanolicus strains was performed with frozen ampules of B. methanolicus as a starter culture. Ampules of B. methanolicus cells were prepared from exponentially growing cultures (OD600 1.0 to 1.5) and stored at -80°C in 15 % (v/v) glycerol [22]. For inoculation, ampules were thawed and 250 μl cell suspension was used to inoculate 100 ml medium. The E. coli strain DH5α was used as a standard cloning host [59]. Recombinant cells were grown in lysogeny broth (LB) medium at 37°C supplemented with ampicillin (100 μg/ml), kanamycin (50 μg/ml), spectinomycin (100 μg/ml), and 1 mM IPTG when appropriate. Recombinant E. coli procedures were performed as described elsewhere [60]. Recombinant protein production was carried out with E. coli BL21 (DE3) as the host [61]. Bacterial strains and plasmids used in this work are listed in Table 1 and oligonucleotides for PCR and cloning are listed in Table 3.

Table 3 List of oligonucleotides used

Homologous overexpression of tkt C and tkt P in B. methanolicus

Overexpression vector pTH1 was used to allow methanol inducible expression of B. methanolicus TKT genes. This vector is analogous to the plasmid pHP13, in which the strong mdh promoter was cloned in-frame with the mdh rbs region to allow methanol inducible expression in B. methanolicus[20, 39]. The DNA fragments of the tkt C and tkt P coding regions were amplified from DNA of B. methanolicus by the primer pair tkt _P-Bme-fw and tkt_ P-Bme-rv, and tkt _C-Bme-fw and tkt _C-Bme-fw (Table 3). The resulting PCR products were digested with Pci I and ligated to the Pci I digested vector pTH1. The resulting vectors were named pTH1-tkt C(Bme) and pTH1-tkt P(Bme), respectively. Crude cell extracts were prepared based on the protocol described elsewhere [20]. B. methanolicus cells were grown in SOB medium with 0.25 mM sucrose to stationary phase (OD600, 2.5 to 3.3). Gene expression was induced by addition of 200 mM methanol at inoculation. 20 ml of the cell culture was harvested by centrifugation (4000 × g, 10 min, 4°C), washed in 50 mM potassium phosphate buffer (pH 7.5) and stored at -20°C. The cells were disrupted by sonication described [29]. Cell debris was removed by centrifugation (14,000 x g, 1 h, 4°C) and the supernatant was collected as crude extract. TKT activity was measured according to assay II.

Purification molecular mass determination of TKT proteins

For protein production with E. coli BL21 (DE3) [61], tkt P and tkt C were amplified by PCR using the primers tkt _C-Xho-fw and tkt _C-Xho-rv and tkt _P-Xho-fw and tkt _P-Xho-rv (Table 3). The resulting PCR products were ligated, after restriction with Xho I, into Xho I restricted pET16b (Novagen, Madison, Wisconsin, USA), resulting in pET16b-tkt C and pET16b-tkt P. The pET16b vector allows the production of an N-terminal decahistidine tagged TKT in E. coli BL21 (DE3). Protein production and purification was performed as described previously [62]. Both enzymes were purified to homogenity. After purification, the His-tag was cleaved by factor Xa (Novagen, San Diego) according to the manufacturer’s recommendations and buffered in 20 mM Tricine, pH 7.7. The protein purification was analyzed by 12% SDS-PAGE [63]. Protein concentration was measured according the method of Bradford using the Bio-Rad Protein-Assay with BSA as standard. The tetrameric structures of the TKT proteins were determined by gel filtration as described previously [62] using 1 mg TKT dissolved in 2 ml of 20 mM Tris–HCl, pH 7.5.

Enzyme assays for the purified TKT proteins

The TKT activity in the direction of S7P + GAP from R5P + Xu5P was done by Assay I, a modified version of a previously described assay [31] using the auxiliary enzymes triose-phosphate isomerase (TPI) and glycerol 3-phosphate dehydrogenase (GPD) from rabbit muscle. The oxidation of NADH was followed setting 1 pmol NADH oxidized equivalent to 1 pmol X5-P consumed. The standard reaction mixture (final volume 1 ml) contained 50 mM Tris–HCl buffer (pH 7.5), 0.25 mM NADH, 2 mM Mn2Cl, 0.4 U/ml TPI, 0.7 U/ml glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and purified TKT protein which was preheated for 3 min at 50°C. NADH reduction (ϵ340nm = 6.22 mM–1 cm–1) was followed at 340 nm on a Shimadzu UV1700 spectrophotometer. The reaction was initiated by the addition of R5-P or X5-P, respectively (final concentration varied between 0.05 - 10 mM). The pH-optimum was defined by using the following buffers (50 mM): acetate (pH 5.0-6.0), phosphate (pH 6.0-7.0), Tris–HCl (pH 7.0-9.0), and glycine-NaOH (pH 9.0-10.0) under standard conditions. The pH was adjusted at 50°C.

Formation of the transketolase apoform and reconstitution of the holoenzyme

Apo-transketolase was obtained by removing the cofactors THDP and divalent cation through dialysis for 24 hours against Tris–HCl buffer pH 7.5 containing 10 mM EDTA. After removing EDTA by dialysis, different divalent cations were tested as possible cofactors in the transketolase reaction using Assay I and 1.25 mM X5-P and R5-P, respectively.

The effect of metal ions and EDTA, ATP or ADP on TKT activity was measured under standard conditions (Assay I) in the presence of Ca2+, Co2+, Cu2+, Mg2, Mn2+ and Ni2+ at 1 mM final concentration in the reaction mixture. The remaining percentage activities were determined by comparison with no metal ion added. To investigate the effect of EDTA, EDTA salt solution was incubated with TKT for 4 minutes. The measurement was done according to standard assay conditions with 1 mM EDTA final concentration in 1 ml reaction mixture. To study the thermal stability of the TKT proteins, the assay mixture described above was prepared in 1.5 ml reaction tubes and incubated for up to 2 h at 30-80°C. Samples were taken periodically and the residual enzyme activity was measured under standard conditions (Assay I) in a separate reaction mixture.

The TKT activity in the direction of E4-P and X5-P from F6-P + GAP was done by Assay II, a modified version of a previously described assay [31] using the auxiliary enzymes Erythrose-4-phosphate dehydrogenase (E4PDH) from E. coli to detect E4-P from the conversion of F6-P and GAP. The oxidation of NADH was followed setting 1 mmol NADH oxidized equivalent to 1 mmol X5-P consumed. The standard reaction mixture (final volume 1 ml) contained 50 mM Tris–HCl buffer (pH 7.5), 0.25 mM NAD+, 2 mM Mn2Cl, 1 mM dithiothreitol (DTT) 2 U/ml E4PDH and purified TKT protein which was preheated for 3 min at 55°C. NAD+ oxidation (ϵ340nm = 6.22 mM–1 cm–1) was followed at 340 nm on a Shimadzu UV1700 spectrophotometer. The reaction was initiated by the addition of GAP or R5-P respectively (final concentration varied between 0.05 - 10 mM).

Hydroxypyruvate (HP) activity (Assay III) was measured by recording the oxidation rate of the α-carbanion intermediate in the presence of ferricyanide according to the method of Joshi and coworkers (2008) [32]. The reaction mixture in 1.0 ml contained 50 mM glycyl-glycine buffer (pH 7.6), 2 mM manganese chloride, 0.2 mM THDP, 0.5 mM potassium ferricyanide, 3 mM F6-P/HP and 0.24 mg enzyme protein. The reaction was initiated by the addition of enzyme and the reduction of ferricyanide was monitored at 420 nm using UV-1700 PC spectrophotometer (Shimadzu, Japan).

DHAS activity was assayed (Assay IV), depending on the purpose of the experiment, by one of three methods described previously [23, 27], with several modifications. For routine assay and to test the effects of glycoaldehyde acceptors on DHAS activity, the activity was measured by a modification of the method of Kato et al. [27] (method A). The reaction mixture (1 ml) contained 50 mmol of standardbuffer (pH 7.0), 0.5 mmol of X5-P, 5 mmol of MgCl2, 0.5 mmol of THDP, 0.16 mmol of NADH, 62.6 U TPI (from baker’s yeast; Sigma Chemical Co.), 0.26 U of a GPD (from rabbit muscle; Sigma), and cell extracts.

To test the effect of glyceraldehyde donors on DHAS activity, the activity was assayed by a method based on the system described by Waits and Quayle [23] (method B). The reaction mixture of method B was the same as that for method A except that the mixture (1 ml) contained 1 mmol ATP and 0.23 U of glycerokinase (from Candida mycoderma; Sigma) instead of TPI. The mixtures for methods A and B were incubated for 90 s to determine endogenous activity. The reaction was started by the addition of 1 mmol of formaldehyde, and the reduction in absorbance at 340 nm (ϵ340 nm = 6.22 mM–1 cm–1) was measured between 75 and 105 s after addition of formaldehyde. One unit of enzyme activity was defined as the amount of enzyme required oxidizing 1 mmol of NADH per min.

Computational analysis

Sequence comparisons were carried out with protein sequences obtained from the NCBI database (http://www.ncbi.nlm.nih.gov), the sequence alignment of the B. methanolicus MGA3 TKT proteins and other TKT was done using CLUSTALW [64] and formatted with Box Shade.

Abbreviations

ADP:

Adenosine diphosphate

ATP:

Adenosine triphosphate

Bme:

B. methanolicus

C:

Chromosomal

DHA:

Dihydroxyacetone

DHAP:

Dihydroxyacetone phosphate

DHAS:

Dihydroxyacetone synthase

DTT:

Dithiothreitol

DXP:

1-deoxy-D-xylulose 5-phosphate Synthase

E4-P:

Erythrose 4-phosphate

Eco:

E. coli

EDTA:

Ethylenediaminetetraacetic acid

F6-P:

Fructose 6-phosphate

FBA:

Fructose 1,6-bisphosphate aldolase

FADH:

Formaldehyde dehydrogenase

FBP:

Fructose 1,6-bisphosphate

FBPase:

Fructose 1,6-bisphosphatase

GAP:

Glyceraldehyde phosphate

GlpX:

Fructose bisphosphatase

HP:

Hydroxypyruvat

HPS:

3-hexulose-6-phosphate synthase

MDH:

Methanol dehydrogenase

P:

Plasmid

PPP:

Pentose phosphate pathway

PHI:

6-phospho-3-hexuloisomerase

R5-P:

Ribose 5-phosphate

RPE:

Ribulose 5-phosphate 3-epimerase

RPI:

Ribose 5-phosphate isomerase

Ru5-P:

Ribulose 5-phosphate

RuMP:

Ribulose monophosphate

S7-P:

Sedoheptulose 7-phosphate

SBA:

Sedoheptulose 1,7-bisphosphate aldolase

SBPase:

Sedoheptulose 1,7-bisphosphatase

TA:

Transaldolase

ThDP:

Thiamine diphosphate

TKT:

Transketolase

X5-P:

Xylulose 5-phosphate.

References

  1. Schenk G, Duggleby RG, Nixon PF: Properties and functions of the thiamin diphosphate dependent enzyme transketolase. Int J Biochem Cell Biol. 1998, 30: 1297-1318. 10.1016/S1357-2725(98)00095-8.

    Article  CAS  PubMed  Google Scholar 

  2. Zhao J, Zhong CJ: A review on research progress of transketolase. Neurosci Bull. 2009, 25: 94-99. 10.1007/s12264-009-1113-y.

    Article  PubMed  Google Scholar 

  3. Breslow R, Appayee C: Transketolase reaction under credible prebiotic conditions. Proc Natl Acad Sci U S A. 2013, 110: 4184-4187. 10.1073/pnas.1301522110.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Datta AG, Racker E: Mechanism of action of transketolase I Properties of the crystalline yeast enzyme. J Biol Chem. 1961, 236: 617-623.

    CAS  PubMed  Google Scholar 

  5. Kochetov GA: Transketolase from yeast, rat liver, and pig liver. Methods Enzymol. 1982, 90 (Kochetov GA): E:209-223.

    Article  Google Scholar 

  6. Kamada N, Yasuhara A, Takano Y, Nakano T, Ikeda M: Effect of transketolase modifications on carbon flow to the purine-nucleotide pathway in Corynebacterium ammoniagenes. Appl Microbiol Biotechnol. 2001, 56: 710-717. 10.1007/s002530100738.

    Article  CAS  PubMed  Google Scholar 

  7. Abe S, Takayarna K, Kinoshita S: Taxonomical studies on glutamic acid producing bacteria. J Gen Appl Microbiol. 1967, 13: 279-301. 10.2323/jgam.13.279.

    Article  Google Scholar 

  8. Villafranca JJ, Axelrod B: Heptulose synthesis from nonphosphorylated aldoses and ketoses by spinach transketolase. J Biol Chem. 1971, 246: 3126-3131.

    CAS  PubMed  Google Scholar 

  9. Masri SW, Ali M, Gubler CJ: Isolation of transketolase from rabbit liver and comparison of some of its kinetic properties with transketolase from other sources Comparative biochemistry and physiology. Comp Biochem Physiol B. 1988, 90: 167-172.

    CAS  PubMed  Google Scholar 

  10. Blass JP, Piacentini S, Boldizsar E, Baker A: Kinetic studies of mouse brain transketolase. J Neurochem. 1982, 39: 729-733. 10.1111/j.1471-4159.1982.tb07953.x.

    Article  CAS  PubMed  Google Scholar 

  11. Mocali A, Paoletti F: Transketolase from human leukocytes Isolation, properties and induction of polyclonal antibodies. Eur J Biochem. 1989, 180: 213-219. 10.1111/j.1432-1033.1989.tb14636.x.

    Article  CAS  PubMed  Google Scholar 

  12. Sprenger GA, Schorken U, Sprenger G, Sahm H: Transketolase A of Escherichia coli K12 Purification and properties of the enzyme from recombinant strains. Eur J Biochem. 1995, 230: 525-532. 10.1111/j.1432-1033.1995.0525h.x.

    Article  CAS  PubMed  Google Scholar 

  13. Kato N, Higuchi T, Sakazawa C, Nishizawa T, Tani Y, Yamada H: Purification and properties of a transketolase responsible for formaldehyde fixation in a methanol-utilizing yeast, candida boidinii (Kloeckera sp) No 2201. Biochim Biophys Acta. 1982, 715: 143-150. 10.1016/0304-4165(82)90352-X.

    Article  CAS  PubMed  Google Scholar 

  14. Ro YT, Eom CY, Song T, Cho JW, Kim YM: Dihydroxyacetone synthase from a methanol-utilizing carboxydobacterium, Acinetobacter sp strain JC1 DSM 3803. J Bacteriol. 1997, 179: 6041-6047.

    PubMed Central  CAS  PubMed  Google Scholar 

  15. Alves AM, Euverink GJ, Hektor HJ, Hessels GI, van der Vlag J, Vrijbloed JW, Hondmann D, Visser J, Dijkhuizen L: Enzymes of glucose and methanol metabolism in the actinomycete Amycolatopsis methanolica. J Bacteriol. 1994, 176: 6827-6835.

    PubMed Central  CAS  PubMed  Google Scholar 

  16. Nakagawa T, Fujimura S, Ito T, Matsufuji Y, Ozawa S, Miyaji T, Nakagawa J, Tomizuka N, Yurimoto H, Sakai Y, Hayakawa T: Molecular characterization of two genes with high similarity to the dihydroxyacetone synthase gene in the methylotrophic yeast Pichia methanolica. Biosci Biotechnol Biochem. 2010, 74: 1491-1493. 10.1271/bbb.100153.

    Article  CAS  PubMed  Google Scholar 

  17. Arfman N, Dijkhuizen L, Kirchhof G, Ludwig W, Schleifer KH, Bulygina ES, Chumakov KM, Govorukhina NI, Trotsenko YA, White D, et al.: Bacillus methanolicus sp nov, a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria. Int J Syst Evol Microbiol. 1992, 42: 439-445.

    CAS  Google Scholar 

  18. Arfman N, Hektor HJ, Bystrykh LV, Govorukhina NI, Dijkhuizen L, Frank J: Properties of an NAD(H)-containing methanol dehydrogenase and its activator protein from Bacillus methanolicus. Eur J Biochem. 1997, 244: 426-433. 10.1111/j.1432-1033.1997.00426.x.

    Article  CAS  PubMed  Google Scholar 

  19. Schendel FJ, Bremmon CE, Flickinger MC, Guettler M, Hanson RS: L-lysine production at 50°C by mutants of a newly isolated and characterized methylotrophic Bacillus sp. Appl Environ Microbiol. 1990, 56: 963-970.

    PubMed Central  CAS  PubMed  Google Scholar 

  20. Brautaset T, Jakobsen OM, Flickinger MC, Valla S, Ellingsen TE: Plasmid-dependent methylotrophy in thermotolerant Bacillus methanolicus. J Bacteriol. 2004, 186: 1229-1238. 10.1128/JB.186.5.1229-1238.2004.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Heggeset TM, Krog A, Balzer S, Wentzel A, Ellingsen TE, Brautaset T: Genome sequence of thermotolerant Bacillus methanolicus: features and regulation related to methylotrophy and production of L-lysine and L-glutamate from methanol. Appl Environ Microbiol. 2012, 78: 5170-5181. 10.1128/AEM.00703-12.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE, Brautaset T: Upregulated transcription of plasmid and chromosomal ribulose monophosphate pathway genes is critical for methanol assimilation rate and methanol tolerance in the methylotrophic bacterium Bacillus methanolicus. J Bacteriol T E. 2006, 188: 3063-3072. 10.1128/JB.188.8.3063-3072.2006.

    Article  CAS  Google Scholar 

  23. Krog A, Heggeset TM, Muller JE, Kupper CE, Schneider O, Vorholt JA, Ellingsen TE, Brautaset T: Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD(+) dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties. PLoS One. 2013, 8: e59188-10.1371/journal.pone.0059188.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. Anthony C: Bacterial oxidation of methane and methanol. Adv Microb Physiol. 1986, 27: 113-210.

    Article  CAS  PubMed  Google Scholar 

  25. de Vries GE, Arfman N, Terpstra P, Dijkhuizen L: Cloning, expression, and sequence analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene. J Bacteriol. 1992, 174: 5346-5353.

    PubMed Central  CAS  PubMed  Google Scholar 

  26. Stolzenberger J, Lindner SN, Wendisch VF: The methylotrophic Bacillus methanolicus MGA3 possesses two distinct fructose 1,6-bisphosphate aldolases. Microbiol. 2013, 159: 1770-1781. 10.1099/mic.0.067314-0.

    Article  CAS  Google Scholar 

  27. Brautaset T, Jakobsen OM, Josefsen KD, Flickinger MC, Ellingsen TE: Bacillus methanolicus: a candidate for industrial production of amino acids from methanol at 50°C. Appl Microbiol Biotechnol. 2007, 74: 22-34. 10.1007/s00253-006-0757-z.

    Article  CAS  PubMed  Google Scholar 

  28. Stolzenberger J, Lindner SN, Persicke M, Brautaset T, Wendisch VF: Characterization of fructose 1,6-bisphosphatase and sedoheptulose 1,7-bisphosphatase from the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus. J Bacteriol. 2013, 195: 5112-5122. 10.1128/JB.00672-13.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Brautaset T, Williams MD, Dillingham RD, Kaufmann C, Bennaars A, Crabbe E, Flickinger MC: Role of the Bacillus methanolicus citrate synthase II gene, citY, in regulating the secretion of glutamate in L-lysine-secreting mutants. Appl Environ Microbiol. 2003, 69: 3986-3995. 10.1128/AEM.69.7.3986-3995.2003.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  30. Iida A, Teshiba S, Mizobuchi K: Identification and characterization of the tktB gene encoding a second transketolase in Escherichia coli K-12. J Bacteriol. 1993, 175: 5375-5383.

    PubMed Central  CAS  PubMed  Google Scholar 

  31. Zhao G, Winkler ME: An Escherichia coli K-12 tktA tktB mutant deficient in transketolase activity requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth. J Bacteriol. 1994, 176: 6134-6138.

    PubMed Central  CAS  PubMed  Google Scholar 

  32. Joshi S, Singh AR, Kumar A, Misra PC, Siddiqi MI, Saxena JK: Molecular cloning and characterization of Plasmodium falciparum transketolase. Mol Biochem Parasitol. 2008, 160: 32-41. 10.1016/j.molbiopara.2008.03.005.

    Article  CAS  PubMed  Google Scholar 

  33. Veitch NJ, Maugeri DA, Cazzulo JJ, Lindqvist Y, Barrett MP: Transketolase from Leishmania mexicana has a dual subcellular localization. Biochem J. 2004, 382: 759-767. 10.1042/BJ20040459.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  34. Stoffel SA, Alibu VP, Hubert J, Ebikeme C, Portais JC, Bringaud F, Schweingruber ME, Barrett MP: Transketolase in Trypanosoma brucei. Mol Biochem Parasitol. 2011, 179: 1-7. 10.1016/j.molbiopara.2011.04.006.

    Article  CAS  PubMed  Google Scholar 

  35. Golbik R, Meshalkina LE, Sandalova T, Tittmann K, Fiedler E, Neef H, Konig S, Kluger R, Kochetov GA, Schneider G, Hubner G: Effect of coenzyme modification on the structural and catalytic properties of wild-type transketolase and of the variant E418A from Saccharomyces cerevisiae. FEBS J. 2005, 272: 1326-1342. 10.1111/j.1742-4658.2005.04562.x.

    Article  CAS  PubMed  Google Scholar 

  36. Hawkins CF, Borges A, Perham RN: A common structural motif in thiamin pyrophosphate-binding enzymes. FEBS Lett. 1989, 255: 77-82. 10.1016/0014-5793(89)81064-6.

    Article  CAS  PubMed  Google Scholar 

  37. Meshalkina L, Nilsson U, Wikner C, Kostikowa T, Schneider G: Examination of the thiamin diphosphate binding site in yeast transketolase by site-directed mutagenesis. Eur J Biochem. 1997, 244: 646-652. 10.1111/j.1432-1033.1997.t01-1-00646.x.

    Article  CAS  PubMed  Google Scholar 

  38. Abedinia M, Layfield R, Jones SM, Nixon PF, Mattick JS: Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase. Biochem Biophys Res Commun. 1992, 183: 1159-1166. 10.1016/S0006-291X(05)80312-2.

    Article  CAS  PubMed  Google Scholar 

  39. Jakobsen OM, Brautaset T, Degnes KF, Heggeset TM, Balzer S, Flickinger MC, Valla S, Ellingsen TE: Overexpression of wild-type aspartokinase increases L-lysine production in the thermotolerant methylotrophic bacterium Bacillus methanolicus. Appl Environ Microbiol. 2009, 75: 652-661. 10.1128/AEM.01176-08.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  40. Kelley-Loughnane N, Biolsi SA, Gibson KM, Lu G, Hehir MJ, Phelan P, Kantrowitz ER: Purification, kinetic studies, and homology model of Escherichia coli fructose-1,6-bisphosphatase. Biochim Biophys Acta. 2002, 1594: 6-16. 10.1016/S0167-4838(01)00261-8.

    Article  CAS  PubMed  Google Scholar 

  41. Stansen C, Uy D, Delaunay S, Eggeling L, Goergen JL, Wendisch VF: Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production. Appl Environ Microbiol. 2005, 71: 5920-5928. 10.1128/AEM.71.10.5920-5928.2005.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  42. Haima P, van Sinderen D, Bron S, Venema G: An improved beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis. Gene. 1990, 93: 41-47. 10.1016/0378-1119(90)90133-C.

    Article  CAS  PubMed  Google Scholar 

  43. Brautaset T, Jakobsen OM, Degnes KF, Netzer R, Naerdal I, Krog A, Dillingham R, Flickinger MC, Ellingsen TE: Bacillus methanolicus pyruvate carboxylase and homoserine dehydrogenase I and II and their roles for L-lysine production from methanol at 50°C. Appl Microbiol Biotechnol. 2010, 87: 951-964. 10.1007/s00253-010-2559-6.

    Article  CAS  PubMed  Google Scholar 

  44. Say RF, Fuchs G: Fructose 1,6-bisphosphate aldolase/phosphatase may be an ancestral gluconeogenic enzyme. Nature. 2010, 464: 1077-1081. 10.1038/nature08884.

    Article  CAS  PubMed  Google Scholar 

  45. Alexander-Kaufman K, Harper C: Transketolase: observations in alcohol-related brain damage research. Int J Biochem Cell Biol. 2009, 41: 717-720. 10.1016/j.biocel.2008.04.005.

    Article  CAS  PubMed  Google Scholar 

  46. Kochetov G, Sevostyanova IA: Binding of the coenzyme and formation of the transketolase active center. IUBMB Life. 2005, 57: 491-497. 10.1080/15216540500167203.

    Article  CAS  PubMed  Google Scholar 

  47. Bobst CE, Tabita FR: The role of cysteine 160 in thiamine diphosphate binding of the Calvin-Benson-Bassham cycle transketolase of Rhodobacter sphaeroides. Arch Biochem Biophys. 2004, 426: 43-54. 10.1016/j.abb.2004.03.027.

    Article  CAS  PubMed  Google Scholar 

  48. Jung EH, Takeuchi T, Nishino K, Itokawa Y: Studies on the nature of thiamine pyrophosphate binding and dependency on divalent cations of transketolase from human erythrocytes. Int J Biochem. 1988, 20: 1255-1259. 10.1016/0020-711X(88)90228-5.

    Article  CAS  PubMed  Google Scholar 

  49. Heinrich PC, Wiss O: Transketolase from human erythrocytes Purification and properties. Helv Chim Acta. 1971, 54: 2658-2668. 10.1002/hlca.19710540829.

    Article  CAS  PubMed  Google Scholar 

  50. Kochetov GA: Transketolase: structure and mechanism of action. Biokhimiia. 1986, 51: 2010-2029.

    CAS  PubMed  Google Scholar 

  51. Wikner C, Nilsson U, Meshalkina L, Udekwu C, Lindqvist Y, Schneider G: Identification of catalytically important residues in yeast transketolase. Biochemistry. 1997, 36: 15643-15649. 10.1021/bi971606b.

    Article  CAS  PubMed  Google Scholar 

  52. Schaaff-Gerstenschlager I, Mannhaupt G, Vetter I, Zimmermann FK, Feldmann H: TKL2, a second transketolase gene of Saccharomyces cerevisiae Cloning, sequence and deletion analysis of the gene. Eur J Biochem. 1993, 217: 487-492. 10.1111/j.1432-1033.1993.tb18268.x.

    Article  CAS  PubMed  Google Scholar 

  53. Schaaff-Gerstenschlager I, Zimmermann FK: Pentose-phosphate pathway in Saccharomyces cerevisiae: analysis of deletion mutants for transketolase, transaldolase, and glucose 6-phosphate dehydrogenase. Curr Genet. 1993, 24: 373-376. 10.1007/BF00351843.

    Article  CAS  PubMed  Google Scholar 

  54. Domain F, Bina XR, Levy SB: Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR. Mol Microbiol. 2007, 66: 383-394. 10.1111/j.1365-2958.2007.05928.x.

    Article  CAS  PubMed  Google Scholar 

  55. Usmanov RA, Kochetov GA: Function of the arginine residue in the active center of baker's yeast transketolase. Biokhimiia. 1983, 48: 772-781.

    CAS  PubMed  Google Scholar 

  56. Usmanov RA, Kochetov GA: Interaction of baker's yeast transketolase modified by 2,3-butanedione with anionic and nonanionic substrates. Biochem Int. 1983, 6: 673-683.

    CAS  PubMed  Google Scholar 

  57. Bystrykh LV, de Koning W, Harder W: Dihydroxyacetone synthase from Candida boidinii KD1. Methods Enzymol. 1990, 188: 435-445.

    Article  CAS  PubMed  Google Scholar 

  58. Esakova OA, Meshalkina LE, Golbik R, Hubner G, Kochetov GA: Donor substrate regulation of transketolase. Eur J Biochem. 2004, 271: 4189-4194. 10.1111/j.1432-1033.2004.04357.x.

    Article  CAS  PubMed  Google Scholar 

  59. Hanahan D: Techniques for transformation of E coli. DNA cloning: a practical approach. Edited by: Glover DM. 1985, Oxford, United Kingdom: IRL Press, 109-135.

    Google Scholar 

  60. Sambrook J, Russell D: Molecular Cloning A Laboratory Manual. 2001, Cold Spring Harbor, NY: Cold Spring Harbor Laboratoy Press, 3

    Google Scholar 

  61. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW: Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990, 185: 60-89.

    Article  CAS  PubMed  Google Scholar 

  62. Lindner SN, Vidaurre D, Willbold S, Schoberth SM, Wendisch VF: NCgl2620 encodes a class II polyphosphate kinase in Corynebacterium glutamicum. Appl Environ Microbiol. 2007, 73: 5026-5033. 10.1128/AEM.00600-07.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  63. Laemmli UK: Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature. 1970, 227: 680-10.1038/227680a0.

    Article  CAS  PubMed  Google Scholar 

  64. Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22: 4673-4680. 10.1093/nar/22.22.4673.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Correspondence to Volker F Wendisch.

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VFW, BM, JS and TB designed the experiments. BM and JS conducted the experiments, analysed the results, and wrote the manuscript. VFW and TB reviewed and revised the manuscript. All authors read and approved the final manuscript.

Benno Markert, Jessica Stolzenberger contributed equally to this work.

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Markert, B., Stolzenberger, J., Brautaset, T. et al. Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus . BMC Microbiol 14, 7 (2014). https://doi.org/10.1186/1471-2180-14-7

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  • DOI: https://doi.org/10.1186/1471-2180-14-7

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