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Table 3 Oligonucleotide primers used in this study

From: The conserved upstream region of lscB/C determines expression of different levansucrase genes in plant pathogen Pseudomonas syringae

Oligonucleotides

Nucleotide sequence (5’ - 3’)†

pe.BC.PG ~ 150 bp

GTCACCCATGCGGGCCAGCAG

lscB_UpN_f

CCCAAGCTTCGATTGCAAGCTGATACACGTACC

lscB_UpN_r

TAGGCTAGCTAGAGGACTATTTTTGAG

lscA_ORF_f

CTAGCTAGCATGAGTAACATCAATTAC

lscA_ORF_r

CCCAAGCTTCGGACGTCATCCTGATCGACAC

lscB_Up_r

TAGGCTAGCAATTGATACCTTTAAATAGCTTTGGGAG

lscA_Up_f

CGGGATCCAGCAAAGCGCTGTAAAACAGG

lscA_Up_r

CTACTAGCTAGCGATGATGTCCTTTATTGGCGC

lscB_ORF_f

GCTCTAGATGTCCACTAGCAGCTCTGCTGTAA

lscB_ORF_r

CCCAAGCTTTCAGTATTACGGATACGATGAGC

lscA_gly_f

TAAGCCCGGATTTTCCGGTC

lscA_gly_r

TACTGTATGCGTGCCGCGTT

lscA_pha_f

TCACGCTGACGGCTGACCGC

lscA_pha_r

GCCTACTGTATGCGTGCCGCG

lscA_syr_f

TCACGCTGACAGCTGATCGC

lscA_syr_r

ACCAACGGTATGCGTACCGC

lscA_tom_f

ATCACCCTGACAGCCGACCG

lscA_tom_r

ACCGACAGTATGTGAACCCCGCT

lscA_f_RT

ATGAGTAACATCAATTACGCACCC

lscA_r_RT

TACTTTGGCAATTGCCGCAC

lscB_f_RT

CTCTGCTGTAAGCCAGCTCAA

lscB_r_RT

CGGGTGTGACGCAGGTGTAA

gyrA_fw

CGAAGAGCTGGAAGTGATCC

gryA_rv

GACGCTGAGCCTGATAGACC

hexR_fw

ATGGACCGCGTAAGAAAC

hexR_rv

TCAGCCTTGATCCTCGATCGG

  1. †Restriction sites in the primers are in italics: GAGCTC – SacI, AAGCTT - HindIII, GCTAGC - NheI, GGATCC - BamHI, TCTAGA – XbaI.