A functional peptidoglycan hydrolase characterized from T4SS in 89K pathogenicity island of epidemic Streptococcus suis serotype 2

Table 1 Bacterial strains and plasmids used in this study

Strains/plasmids	Relevant characteristics^*	Source/reference
Strains
*S. suis*
05ZYH33	A highly virulent strain isolated from a dead patient with STSS	Lab collection
ΔvirB1-89K	An isogenic virB1-89K mutant of strain 05ZYH33; Spc^r	[12]
CΔvirB1-89K	Complemented strain of ΔvirB1-89K; Spc^r; Cm^r	[12]
*M. lysodeikticus*
ATCC4698	Suitable for substrate for the assay of lysozyme	Sigma-Aldrich
*E. coli*
DH5α	Cloning host for maintaining the recombinant plasmids	Lab collection
BL21(DE3)	Expression host for exogenous protein production	Lab collection
Plasmids
pMD19-T	Cloning vector; Amp^r	TaKaRa
pET-21a(+)	His-tag fusion expression vector; Amp^r	Novagen
pET21a-CHAP	A recombinant vector with the background of pET-21a(+), designed for expression of the CHAP domain of VirB1-89K; Amp^r	This work

^*Amp^r, ampicillin resistant; Cm^r, chloramphenicol resistant; Spc^r, spectinomycin resistant.

ISSN: 1471-2180