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Figure 7 | BMC Microbiology

Figure 7

From: Identification of a predicted partner-switching system that affects production of the gene transfer agent RcGTA and stationary phase viability in Rhodobacter capsulatus

Figure 7

In vitro interaction between RbaW and RbaV. Pull-down assays were done using NHS bead-conjugated recombinant RbaW supplemented with recombinant RbaV or control protein (lysozyme). Conjugated control beads (Lanes 1 and 2) were not supplemented with test protein while non-conjugated bead controls (Lanes 3 and 6) were blocked by 100 mM Tris. Both N- and C-terminal 6x-His-tagged RbaW proteins were conjugated and tested against N-terminal 6x-His-tagged RbaV (Lanes 4 and 5, respectively). Lysozyme (14.3 kDa) was tested against RbaW-conjugated beads (Lanes 7 and 8) as a control. The gel was stained with Coomassie blue and the resulting image was adjusted for brightness and contrast. Molecular weight references are indicated on the left of the gel.

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BMC Microbiology

ISSN: 1471-2180

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