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Table 6 C. jejuni proteins responding to decreased iron concentration assessed by comparing protein expression after growth on MH + blood and on MH medium

From: The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts

Protein

Locus in NCTC11168

GI number

Log2change in MH medium

   

00-2425

00-2426

Iron acquisition

    

Iron-uptake ABC transporter ATP-binding protein CfbpC, putative

Cj0173c

gi|218561852

1.27 ± 0.75

1.10 ± 0.53

Iron ABC transporter, permease CfbpB, putative

Cj0174c

gi|121612175

0.80 ± 0.71

0.90 ± 0

Iron-uptake ABC transporter, periplasmic iron-binding protein CfbpA, putative

Cj0175c

gi|218561854

2.00 ± 1.30

1.97 ± 1.27

Iron transport protein, putative

Cj0177

gi|218561856

3.30 ± 0.17

2.73 ± 1.05

TonB-denpendent outer membrane receptor, putative

Cj0178

gi|218561857

2.70 ± 0.56

2.97 ± 0.29

Biopolymer transport ExbB protein, TonB transport system

Cj0179

gi|218561858

3.03 ± 0.32

3.37 ± 1.61

Periplasmic TonB transport protein

Cj0753c

gi|315124276

3.5 (single value)

3.5 (single value)

Ferric receptor CfrA

Cj0755

gi|57237601

2.37 ± 0.15

2.23 ± 0.81

Enterochelin uptake ATP-binding protein CeuD

Cj1354

gi|218562963

2.00 ± 1.06

2.00 ± 0.56

Enterochelin ABC transporter, periplasmic enterochelin-binding protein CeuE

Cj1355

gi|121612138

1.80 ± 0.66

1.40 ± 0.26

Periplasmic protein P19

Cj1659

gi|157415879

1.97 ± 1.50

2.10 ± 1.04

Iron permease, putative

Cj1658

gi|218563246

1.97 ± 1.04

1.70 ± 1.01

Heme iron utilization protein, putative

Cj1613c

gi|121612296

3.60 ± 0.95

3.70 ± 0.5

TonB-dependent heme receptor ChuA

Cj1614

gi|57238627

3.35 ± 0.35

4.20 ± 0

TonB-dependent heme receptor ChuA

Cj1614

gi|157415833

1.80 ± 0.70

1.73 ± 0.87

TonB-dependent heme receptor ChuA

Cj1614

gi|121613006

1.20 ± 0.56

0.33 ± 0.64

Hemin ABC transporter, permease protein, putative ChuB

Cj1615

gi|153951720

3.17 ± 0.55

2.93 ± 1.19

Hemin uptake system ATP-binding protein ChuC , putative

Cj1616

gi|218563205

2.97 ± 0.74

3.07 ± 0.50

Hemin uptake system periplasmic hemin-binding protein, putative

Cj1617

gi|218563206

2.83 ± 0.59

2.53 ± 0.42

Biopolymer transport ExbD protein, TonB transport system

Cj1629

gi|153952038

1.63 ± 0.81

1.57 ± 0.49

Integral membrane protein, putative

Cj1660

gi|218563248

1.27 ± 0.81

1.5 ± 0.79

ABC transporter permease, putative

Cj1661

gi|218563249

1.67 ± 0.97

1.57 ± 0.67

Integral membrane protein, putative

Cj1662

gi|218563250

1.93 ± 1.15

1.60 ± 0.87

ABC transporter ATP-binding protein

Cj1663

gi|157415883

1.53 ± 0.80

1.53 ± 0.51

Oxidative stress

    

Thioredoxin reductase

Cj0146c

gi|218561827

0.93 ± 0.47

0.87 ± 0.5

Antioxidant, AhpC/Tsa family

Cj0334

gi|157414631

0.97 ± 0.32

0.70 ± 0.20

Catalase

Cj1385

gi|121612952

3.37 ± 0.23

2.80 ± 0.56

Thioredoxin-like protein

Cj1664

gi|121613144

1.23 ± 0.91

1.27 ± 1.08

Anthranilate synthesis

    

Anthranilate synthase component I

Cj0345

gi|121612335

0.80 ± 0.75

0.87 ± 0.71

Anthranilate synthase component II

Cj0346

gi|121612640

0.67 ± 0.60

0.60 ± 0.46

N-(5′phosphoribosyl)anthranilate isomerase

Cj0347

gi|157414644

1.00 ± 0.53

0.83 ± 0.65

Chemotaxis

    

Methyl-accepting chemotaxis protein

Cj1189c

gi|121613242

0.70 ± 1.10

0.07 ± 0.06

Methyl-accepting chemotaxis protein

Cj1506c

gi|121613017

2.0 ± 1.31

3.23 ± 2.40

Methionine biosynthesis

    

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase MetE

Cj1201

gi|157415465

0.50 ± 0.35

1.20 ± 0.26

Homoserine O-succinyltransferase MetA

Cj1726c

gi|121613233

0.80 ± 0.35

1.53 ± 0.55

Homoserine O-acetyltransferase MetB

Cj1727c

gi|315125108

1.27 ± 0.57

1.93 ± 0.9

Molybdate transport

    

Molybdate transport system ATP-binding protein

Cj0300c

gi|157414597

0.67 ± 0.15

0.77 ± 0.25

Hypothetical protein C8J_0279 molybdenum-pterin-binding protein

Cj0302

gi|157414599

0.30 (single value)

0.67 ± 0.21

Molybdate transport system substrate-binding protein

Cj0303

gi|157414600

0.83 ± 0.75

0.77 ± 0.25

Hypothetical proteins

    

Hypothetical protein C8J_0062

Cj0069

gi|157414383

−0.63 ± 0.06

−0.60 ± 0.17

Hypothetical protein CJJ81176_0110

Cj0073c

gi|121613178

−0.93 ± 0.68

−0.57 ± 0.35

Hypothetical protein C8J_0189

Cj0200c

gi|157414509

0.67 ± 0.81

0.80 ± 0.78

Hypothetical protein Cj0427

Cj0427

gi|218562085

−1.47 ± 0.67

0.07 ± 0.58

Hypothetical protein CJJ81176_0676

Cj0648

gi|121613289

0.70 ± 0.28

0.50 ± 0.36

Hypothetical protein C8J_1185

Cj1242

gi|157415505

−0.60 (single value)

−1.10 ± 0.71

Hypothetical protein C8J_1529

Cj1627c

gi|157415847

0.60 ± 0.20

0.50 ± 0.61

Hypothetical protein CJJ81176_1628

Cj1637c

gi|121613390

−0.33 ± 0.15

−0.73 ± 0.35

Hypothetical protein C8J_1561

Cj1659

gi|157415879

1.97 ± 1.50

2.10 ± 1.04

Hypothetical protein CJE0598 CJIE2 prophage

not present

gi|57238304

1.10 ± 0.28

0.77 ± 0.40

Hypothetical protein CXIVA_22050

not present

gi|339443269

0.67 ± 0.57

0.50 ± 0.53

Transporters

    

Sodium/dicarboxylate symporter

Cj0025c

gi|121612756

0.80 ± 0.66

0.40 ± 0.44

Anaerobic C4-dicarboxylate transporter

Cj0088

gi|218561769

−1.10 ± 0.61

−0.63 ± 0.23

OPT family oligopeptide transporter

Cj0204

gi|121613125

0.73 ± 0.21

0.50 ± 0.17

Other function or unknown function

    

Non-haem iron protein

Cj0012c

gi|218561705

−0.83 ± 0.61

−0.77 ± 0.64

Cytochrome c family protein

Cj0037c

gi|121612292

0.93 ± 0.21

1.03 ± 0.35

Aspartate ammonia-lyase

Cj0087

gi|157414400

−1.10 ± 0.50

−1.1 ± 0.40

Periplasmic protein, putative

Cj0093

gi|218561774

0.80 ± 0.53

0.80 ± 0.56

Superoxide dismutase, Fe

Cj0169

gi|157414485

−1.23 ± 0.84

−1.20 ± 1.47

Superoxide dismutase, Fe

Cj0169

gi|153951398

−3.70 ± 2.95

−0.93 ± 0.97

Integral membrane protein

Cj0236c

gi|153952171

0.53 ± 0.75

0.97 ± 0.76

Chemotaxis histidine kinase

Cj0284c

gi|218561946

−0.83 ± 0.61

−0.53 ± 0.42

Ferredoxin 4Fe-4S

Cj0333c

gi|121612302

−1.10 ± 0.89

0.53 ± 0.15

Tryptophan synthase subunit alpha

Cj0349

gi|121613100

0.67 ± 0.47

0.80 ± 0.56

Cytochrome c551 peroxidase

Cj0358

gi|121612870

−0.67 ± 0.55

0.50 ± 0.17

Putative integral membrane protein

Cj0421c

gi|218562079

1.00 ± 0.71

0.73 ± 0.12

Putative periplasmic protein

Cj0425

gi|218562083

1.70 (single value)

1.10 (single value)

Succinate dehydrogenase flavoprotein subunit

Cj0437

gi|218562095

−0.70 ± 0.44

−0.6 ± 0.87

DNA polymerase III subunit epsilon

Cj0452

gi|218562107

−0.23 ± 0.21

−0.67 ± 0.31

Thiamine biosynthesis protein ThiC

Cj0453

gi|121613325

−2.23 ± 0.38

−1.83 ± 0.32

ATP-dependent chaperone protein ClpB

Cj0509c

gi|121613623

−0.33 ± 0.15

−0.67 ± 0.15

2-oxoglutarate-acceptor oxidoreductase subunit OorD

Cj0535

gi|157414816

−0.87 ± 0.55

0.63 ± 0.66

2-oxoglutarate-acceptor oxidoreductase subunit OorA

Cj0536

gi|157414817

−0.73 ± 0.32

−0.67 ± 0.15

Aminohydrolase family protein, putative

Cj0556

gi|218562208

0.90 ± 0.57

1.13 ± 0.71

Quinol dehydrogenase membrane component NapH

Cj0782

gi|121612888

−0.67 ± 0.49

−0.55 ± 0.35

Bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Cj0835c

gi|218562463

−1.00 ± 0.26

−0.93 ± 0.59

Thermonuclease family protein

Cj0979c

gi|121612563

−0.83 ± 0.38

−0.73 ± 0.68

Amino acid transporter, periplasmic solute-binding protein, putative

Cj0982c

gi|218562598

1.03 ± 0.38

0.77 ± 0.4

Arginyl-tRNA-protein transferase

Cj1035c

gi|121613462

−0.73 ± 0.29

−0.60 ± 0.26

Flippase

Cj1130c

gi|218562744

−0.27 ± 0.38

−0.67 ± 0.42

Cytochrome c553

Cj1153

gi|157415419

−0.77 ± 0.25

−0.53 ± 0.38

Cyclopropane fatty acid biosynthesis

Cj1183c

gi|57238055

1.07 ± 0.67

0.73 ± 0.32

NLPA family lipoprotein, putative

Cj1200

gi|218562812

0.47 ± 0.83

1.20 ± 0.56

Hemerythrin family non-heme iron protein

Cj1224

gi|121612625

−0.73 ± 0.25

−0.07 ± 0.21

Quinone-reactive Ni/Fe-hydrogenase, small subunit

Cj1267c

gi|157415531

−0.63 ± 0.46

−0.57 ± 0.21

Endoribonuclease L-PSP, putative

Cj1388

gi|218562997

0.77 ± 0.21

0.40 ± 0.36

Putative Ni/Fe-hydrogenase small subunit

Cj1399c

gi|218563003

0.73 ± 0.31

0.57 ± 0.15

Hypothetical protein C8J_1356 ATP/GTP-binding protein

Cj1450

gi|157415675

−0.87 ± 0.38

−0.80 ± 0.46

Transformation system protein CtsX

Cj1472

gi|121612126

−0.55 ± 0.7

−1.20 (single value)

Transformation system protein CtsP

Cj1473c

gi|121613067

−0.65 ± 0.07

−0.13 ± 0.31

Pyruvate-flavodoxin oxidoreductase

Cj1476c

gi|315124909

−0.87 ± 0.38

0.63 ± 0.42

Formate dehydrogenase, iron-sulfur subunit

Cj1510c

gi|157415732

−0.73 ± 0.71

−0.73 ± 0.45

Dephospho-CoA kinase

Cj1530

gi|218563123

0.73 ± 0.68

0.57 ± 0.74

Multidrug transporter membrane component/ATP-binding component

Cj1587c

gi|121613099

1.40 ± 0.53

1.00 ± 0.44

Two-component regulator (DNA binding response regulator), putative

Cj1608

gi|218563197

−0.07 ± 0.31

−2.50 ± 1.65

Citrate synthase

Cj1682c

gi|157415898

−0.80 ± 0.36

−0.73 ± 0.21

50S ribosomal protein L2

Cj1704c

gi|121612340

−0.47 ± 0.85

−0.80 ± 0.46

2-isopropylmalate synthase

Cj1719c

gi|121612265

0.83 ± 0.68

0.67 ± 0.21

Hypothetical protein CJE0598 CJIE2 prophage

CJE0598

gi|57238304

1.10 ± 0.28

0.77 ± 0.4

Flagellin

CJE1526

gi|57238729

−0.87 ± 0.25

−0.80 ± 0.44