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Table 1 Proteins regulated with ≥0.6 log 2 change in three isolates carrying CJIE1 versus the isolate without CJIE1 when grown on MH + 10% sheep blood

From: The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts

Protein name

GI number

Locus in NCTC11168a

Protein function

log2changeb

CJIE1 phage repressor protein CJE0215

gi|57237226

Not present

Maintenance of lysogeny

3.10 ± 1.27*

CJIE1 hypothetical protein CJE0246

gi|315124241

Not present

CJIE1 prophage capsid protein

2.95 ± 0.07*

CJIE1 00–2425 ORF 11

gi|313116388

Not present

Unknown

2.67 ± 0.65

CJIE1 extracellular deoxyribonuclease CJE0256

gi|57237266

Not present

Degradation of DNA

2.3 ± 0.53

CJIE1 phage major tail tube protein CJE0226

gi|315124254

Not present

Prophage structural protein

2.20 ± 0*

CJIE1 major tail sheath protein CJE0227

gi|315124253

Not present

Prophage structural protein

2.20 (single value)

CJIE1 signal peptidase I associated with PanB

gi|315124225

Not present

Prophage repressor

1.57 ± 0.78

CJIE1 hypothetical protein ICDCCJ07001_659 ORF7

gi|315124224

Not present

Unknown

2.95 ± 0.35*

Flagellar protein FlaG

gi|157414828

Cj0547

Flagellar protein

−0.77 ± 0.76

Flagellar protein FliS

gi|121612919

Cj0549

Flagellin specific chaperone

−1.00 ± 0.44

Invasion phenotype protein CipA

gi|157414972

Cj0685c

Sugar transferase

2.97 ± 0.40

Phosphate acetyltransferase Pta

gi|121613717

Cj0688

Acetate metabolism, regulation

2.30 ± 0.26

Acetate kinase AckA

gi|57236992

Cj0689

Acetate metabolism, regulation

2.00 ± 0.26

Integral membrane protein CstA

gi|218562536

Cj0917c

Carbon starvation protein

−0.73 ± 0.42

Aspartate carbamoyltransferase catalytic subunit PyrB

gi|218562712

Cj1098

Pyrimidine biosynthesis

1.66 ± 0.10

Oligoendopeptidase F PepF

gi|218562713

Cj1099

Degradation of macromolecules

1.73 ± 0.31

Hypothetical protein CJJ81176_1118

gi|121613077

Cj1100

Unknown

1.50 ± 0.44

ATP-dependent DNA helicase UvrD

gi|121612511

Cj1101

DNA helicase

1.70 ± 0.35

Hypothetical protein CJE1504

gi|57238362

Cj1305c

Unknown

−0.93 ± 0.4

Hypothetical protein ICDCCJ07001_1249

gi|315124755

Cj1305c

Unknown

−2.70 ± 0.85*

Methyltransferase protein

gi|218563030

Cj1426c

Part of capsule locus

−1.23 ± 0.38

Hypothetical protein Cj1429c

gi|218563033

Cj1429c

Capsule biosynthesis

1.73 ± 1.33

  1. aGI numbers were retrieved by the Mascot software used for peptide identification; loci in NCTC 11168 were found by blastp searches by searching the NCBI non-redundant protein database using the protein sequence associated with the GI number.
  2. bThree replicate experiments were done. Some experiments returned values for the protein in only 2/3 experiments; these are marked with a ‘*’. Otherwise the mean + SD log2 change was calculated using all three values. Experiments in which a protein expression level was identified in only one or three biological replicates are indicated with the term “single value”. Positive values indicate that proteins were upregulated in isolates carrying CJIE1 and negative values indicate proteins were downregulated in isolates carrying CJIE1 when compared with isolate 00–2426 lacking the CJIE1 prophage.