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Table 1 Proteins regulated with ≥0.6 log 2 change in three isolates carrying CJIE1 versus the isolate without CJIE1 when grown on MH + 10% sheep blood

From: The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts

Protein name GI number Locus in NCTC11168a Protein function log2changeb
CJIE1 phage repressor protein CJE0215 gi|57237226 Not present Maintenance of lysogeny 3.10 ± 1.27*
CJIE1 hypothetical protein CJE0246 gi|315124241 Not present CJIE1 prophage capsid protein 2.95 ± 0.07*
CJIE1 00–2425 ORF 11 gi|313116388 Not present Unknown 2.67 ± 0.65
CJIE1 extracellular deoxyribonuclease CJE0256 gi|57237266 Not present Degradation of DNA 2.3 ± 0.53
CJIE1 phage major tail tube protein CJE0226 gi|315124254 Not present Prophage structural protein 2.20 ± 0*
CJIE1 major tail sheath protein CJE0227 gi|315124253 Not present Prophage structural protein 2.20 (single value)
CJIE1 signal peptidase I associated with PanB gi|315124225 Not present Prophage repressor 1.57 ± 0.78
CJIE1 hypothetical protein ICDCCJ07001_659 ORF7 gi|315124224 Not present Unknown 2.95 ± 0.35*
Flagellar protein FlaG gi|157414828 Cj0547 Flagellar protein −0.77 ± 0.76
Flagellar protein FliS gi|121612919 Cj0549 Flagellin specific chaperone −1.00 ± 0.44
Invasion phenotype protein CipA gi|157414972 Cj0685c Sugar transferase 2.97 ± 0.40
Phosphate acetyltransferase Pta gi|121613717 Cj0688 Acetate metabolism, regulation 2.30 ± 0.26
Acetate kinase AckA gi|57236992 Cj0689 Acetate metabolism, regulation 2.00 ± 0.26
Integral membrane protein CstA gi|218562536 Cj0917c Carbon starvation protein −0.73 ± 0.42
Aspartate carbamoyltransferase catalytic subunit PyrB gi|218562712 Cj1098 Pyrimidine biosynthesis 1.66 ± 0.10
Oligoendopeptidase F PepF gi|218562713 Cj1099 Degradation of macromolecules 1.73 ± 0.31
Hypothetical protein CJJ81176_1118 gi|121613077 Cj1100 Unknown 1.50 ± 0.44
ATP-dependent DNA helicase UvrD gi|121612511 Cj1101 DNA helicase 1.70 ± 0.35
Hypothetical protein CJE1504 gi|57238362 Cj1305c Unknown −0.93 ± 0.4
Hypothetical protein ICDCCJ07001_1249 gi|315124755 Cj1305c Unknown −2.70 ± 0.85*
Methyltransferase protein gi|218563030 Cj1426c Part of capsule locus −1.23 ± 0.38
Hypothetical protein Cj1429c gi|218563033 Cj1429c Capsule biosynthesis 1.73 ± 1.33
  1. aGI numbers were retrieved by the Mascot software used for peptide identification; loci in NCTC 11168 were found by blastp searches by searching the NCBI non-redundant protein database using the protein sequence associated with the GI number.
  2. bThree replicate experiments were done. Some experiments returned values for the protein in only 2/3 experiments; these are marked with a ‘*’. Otherwise the mean + SD log2 change was calculated using all three values. Experiments in which a protein expression level was identified in only one or three biological replicates are indicated with the term “single value”. Positive values indicate that proteins were upregulated in isolates carrying CJIE1 and negative values indicate proteins were downregulated in isolates carrying CJIE1 when compared with isolate 00–2426 lacking the CJIE1 prophage.