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Table 3 List of oligonucleotides used

From: Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus

Name

Sequence (5’-3’)

pET16b_Fw

GCTAACGCAGTCAGGCACCGTGTA

pET16b_Rv

GACTCACTATAGGGGAATTGTGAGCG

tktC_Fw_XhoI

CCGGCTCGAG TTG TTTGATAAAATTGACCAT

tktC_Rv_XhoI

CCGGCTCGAG TTA TTGTTTAAGTAAAGCT

tktP_Fw_XhoI

GCGCCTCGAG GTG CTCCAACAAAAAATAGAT CG

tktP_Rv_XhoI

GGCGCTCGAG TTA GAGAAGCTTTTTAAAATGAGAAA

tkt_C_Seq1

GCGTCATTTGGCAGCGGTATATAAT

tkt_C_Seq2

TCTAGGTCCTGAAGAACGAAAGC

tkt_C_Seq3

GGCTCGGCAGATCTTGCTAGTTC

tkt_P_Seq1

CCCTCATACGCTTTTTCAGAATC

tkt_P_Seq2

GCTAGAGCATTTAACACTGCACC

tkt_P_Seq3

CGATCTTGAACACTCTCACTAAATG

gapb_fw

GCGACTCGAG ATG ACCGTACGCGTAGCGATAA

gapb_rv

GCGTCTCGAG TTA CCTGAAAGCAACAGTAGC

  1. Restriction sites are highlighted in italics, stop and start codons are underlined.