Taylorellae exhibit low cytotoxicity to A. castellanii . Acanthamoeba castellanii were infected with E. coli, T. equigenitalis, T. asinigenitalis or L. pneumophila with an MOI of 50. The viability of amoebic cells in infected monolayers was quantified at an indicated time using Alamar blue dye. These data are representative of two independent experiments done in triplicate. Each bar represents the mean of triplicate wells; error bars represent the standard deviations.