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Table 3 Identities of proteins purified by glutathione-affinity purification of cell lysates prepared from cultured wild type and transformant Z. mobilis ATCC 29191 strains

From: pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis

Expression vector used

Z. mobilis proteins identified

Corresponding locus tag (gene)

-

Glyoxalase/bleomycin resistance protein/dioxygenase Glo

ZZ6_1397 (glo)

Glutathione S-transferase domain protein

ZZ6_0208

pZ7-GST

Glyoxalase/bleomycin resistance protein/dioxygenase Glo

ZZ6_1397 (glo)

Recombinant GST (from expression vector)

-

Glutathione S-transferase domain protein

ZZ6_0208

pZ7-GST-acpP

Glyoxalase/bleomycin resistance protein/dioxygenase Glo

ZZ6_1397 (glo)

Holo-acyl-carrier-protein synthase AcpS

ZZ6_1409 (acpS)

(3R)-hydroxymyristoyl-ACP dehydratase FabZ

ZZ6_0182 (fabZ)

Glutathione S-transferase domain protein

ZZ6_0208

Acyl carrier protein AcpP

ZZ6_0066 (acpP)

Glyceraldehyde-3-phosphate dehydrogenase G3P

ZZ6_1034 (gap)

Pyruvate decarboxylase PDC

ZZ6_1712 (pdc)

pZ7-GST-kdsA

Glyoxalase/bleomycin resistance protein/dioxygenase Glo

ZZ6_1397 (glo)

Glutathione S-transferase domain protein

ZZ6_0208

2-dehydro-3-deoxyphosphooctonate aldolase KdsA

ZZ6_1604 (kdsA)

Translation elongation factor Ts

ZZ6_0173 (tsf)

Translation elongation factor Tu

ZZ6_0750 (tuf)

CTP synthase PyrG

ZZ6_0800 (pyrG)

 

Chaperone protein DnaK

ZZ6_0619 (dnaK)