Table 1 Strains and plasmids used in this study

Escherichia coli

     DH10B

F^- mcrA ∆(mrr-hsdRMS-mcrBC) Ф80dlacZ∆M15 ∆lacX74 deoR recA1 endA1 araD139 ∆(ara-leu)7649 galU galK rspL nupG

Invitrogen

     BL21 (DE3)

E. coli B dcm ompT hsdS(rB-mB-) gal

Stratagene

Zymomonas mobilis subsp. mobilis

     NCIMB 11163

Wild-type strain

NCIMB, C. Drainas

     CU1 Rif2

Mutant strain of ATCC 10988

C. Drainas [20]

     ATCC 29191

Wild-type strain, DSM 3580, z6, NCIMB 11199

DSMZ

Plasmids

     pACYC184

cm ^r, tet ^r, ori (p15A)

NEB

     pUC18

amp ^r, ori (pMB1)

Stratagene

     pGEX4T1

amp ^r, ori (pMB1), lacI, P_tac-gst-Term

GE Healthcare

     Cm-pUC18

cm ^r, ori (pMB1)

This study

     pUCZM-1

cm ^r, rep (pZMO1A), ori (pZMO1A), ori (pMB1)

This study

     pUCZM-3

cm ^r, rep (pZMO7), ori (pZMO7), ori (pMB1)

This study

     pZ7-184

cm ^r, rep (pZMO7), ori (pZMO7), ori (p15A)

This study

     pZ7C

cm ^r, rep (pZMO7), ori (pZMO7), ori (pMB1)

This study

     pZ7-GST

cm ^r, rep (pZMO7), ori (pZMO7), P_tac-gst-Term, ori (pMB1)

This study

     pZ7-GST-acpP

cm ^r, rep (pZMO7), ori (pZMO7), P_tac-gst-acpP-Term, ori (pMB1)

This study

     pZ7-GST-dnaJ

cm ^r, rep (pZMO7), ori (pZMO7), P_tac-gst-dnaJ-Term, ori (pMB1)

This study

     pZ7-GST-hfq

cm ^r, rep (pZMO7), ori (pZMO7), P_tac-gst-hfq-Term, ori (pMB1)

This study

     pZ7-GST-holC

cm ^r, rep (pZMO7), ori (pZMO7), P_tac-gst-holC-Term, ori (pMB1)

This study

     pZ7-GST-kdsA

cm ^r, rep (pZMO7), ori (pZMO7), P_tac-gst-kdsA-Term, ori (pMB1)

This study

     ppk2-TOPO

amp ^r ,kan ^r , ori (ColE1), P_lac-lacZα, ori (f1)

This study