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Table 3 Spa -typing of S. aureus strains from a single individual AE with two sets of primers: standard primers 1095 F/1517R and novel primers spaT3-F/1517R

From: Prevalence of Staphylococcus aureus protein A (spa) mutants in the community and hospitals in Oxfordshire

Time points, months

DNA prep (mixed boilate)

12 single colony picks2

 

1095 F/1517R

spaT3-F/1517R

spaT3-F/1517R

AE-0

t230

MST1

t230/t012

AE-1

non-typable

t012

t012

AE-2

t230

MST

t230/t012

AE-4

t230

MST

t230

AE-6

t230

MST

t230/t528

AE-8

t008

MST

t008/t012/t571

AE-10

t230

MST

t230

AE-12

t230

MST

t230

AE-14

t230

MST

t230

  1. 1mixed sequence traces; 2 spa-types in bold have delE and could not be typed with standard primers; spa-repeats:
  2. t230: 08-16-02-16-34.
  3. t571: 08-16-02-25-02-25-34-25.
  4. t008: 11-19-12-21-17-34-24-34-22-25.
  5. t012: 15-12-16-02-16-02-25-17-24-24.
  6. t528: 04.