Amylase production by Preussia minima, a fungus of endophytic origin: optimization of fermentation conditions and analysis of fungal secretome by LC-MS

Zaferanloo, Bita; Bhattacharjee, Shatabdi; Ghorbani, Mahmood M; Mahon, Peter J; Palombo, Enzo A

doi:10.1186/1471-2180-14-55

Table 3 Proteins in the secretome of Preussia minima identified by LC MS/MS and compared to proteins in the NCBI database ¹ by BLAST searching

From: Amylase production by Preussia minima, a fungus of endophytic origin: optimization of fermentation conditions and analysis of fungal secretome by LC-MS

Spot No	Enzyme category, protein name/function²	Target substrate	Species	Accession No.	Mascot score
1	Xylanse	Xylan	Bacillus subtilis	gi 139865	65
2	Chitinase	Chitin	Bacillus subtilis	gi 16079742	59
3	Pectate lyase	Pectin	Bacillus subtilis	gi 16080548	72
4	Phosphoribosyl amino imidazole Carboxylase catalytic subunit		Legionella lonfbeachae	gi 270158584	76
5	Natto kinase		Bacillus subtilis	gi 14422313	66
6	Protease	Protein	Xenopus tropicalis	gi 58332100	51
7	Alpha amylase	Starch	Bacillus subtilis	gi 255767082	85
8	Cellulase	Cellulose	Bacillus subtilis	gi 39777	67
9	Nicotinate-Nucleotide pyrophosphorylase		Bacillus subtilis	gi 16079838	77
10	Endo 1,4- beta mannosidase	Mannan	Bacillus amyloliquefaciens	gi 384158076	52
11	Beta- 1,4-mannase	Mannan	Bacillus pumilus	gi 347311566	82
12	Endo-beta 1,4 mannase	Mannan	Bacillus subtilis	gi 84688836	396
13	Mannase	Mannan	Bacillus licheniformis	gi 239634423	73
14	Hypothetical protein		Zymoseptoria tritici	gi 398404980	54
15	Bacillo peptidase	Protein	Bacillus subtilis	gi 62946514	125
16	Hypothetical protein		Colletotrichum	gi 429850915	67
17	Arginase	Xylan	Bacillus subtilis	gi 16081084	87
18	Arabian-endo1,5-alpha-L-arabinase	Xylan	Bacillus subtilis	gi 1770013	118
19	Neutral protease	Protein	Bacillus mesenterious	gi 56405351	69
20	Bacillo peptidase F	Protein	B. mesenterious	gi 142609	110
21	Unnamed protein product		Cyanophora paradoxa	gi 11416	69
22	Endo-1,4-beta-glucanase	Xyloglucan	Bacillus subtilis	gi 16079934	80
23	Catalase	Hydrogen peroxide	Aspergillus fumigatus	gi 1857716	216
24	VBS		Aspergillus flavus	gi 46370484	70
25	Aldose 1- epimerase	Glucose	Pyrenophora tritici	gi 189190	81
26	Alpha amylase	Starch	Magnaporthe oryzae	gi 389646691	56
27	Hypothetical protein		Gibberella zea	gi 46126833	84
28	Extracellular neutral metalloprotease	Protein	Bacillus subtilis	gi 160789534	77
29	Catalase	Hydrogen peroxide	Glomerella graminicola	gi 310791536	107
30	Cellobiose dehydrogenase	Cellulose	Cochliobolus sativus	gi 451856	92
31	Hypothetical protein		Aspergillus terreus	gi 115388337	78
32	Mycelial catalase	Hydrogen peroxide	Neosartorya fischeri	gi 119474019	105
33	Hypothetical protein SMAC_03015		Sordaria macrospora	gi\|336269335	68

¹Assignment of proteins to all the entries in the NCBI database (http://www.ncbi.nlm.nih.gov/) was determined by ESI-LC-MS/MS of protein spots or bands of 1D and 2D gels. Identification.
²Names or predicted functions of conserved domains in proteins in the NCBI nr database (http://www.ncbi.nlm.nih.gov/) to which significant peptide matches (P < 0.05) were made using the Mascot search engine. The enzyme in bold (spot 26) denotes the 70 kDa α-amylase described above.

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ISSN: 1471-2180

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