Effect of pH and temperature on specific amylase activity of EL-14 and standard. (A) EL-14 was grown at different pH and specific amylase activity was determined. (B) EL-14 was grown at different temperatures and specific amylase activity was determined. (C) standards (A. oryzae and A. niger) were grown at the same conditions to compare their activity with P. minima. The crude extracellular sample (EL-14) exhibited the highest activity at 25°C and pH 9, significantly more than standard. Means followed by the same letter within a column are not significantly different at P < 0.01 according to the Duncan multiple range test.