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Table 3 Phosphoglycerate kinase specific activity (U/mg)

From: Characterization of 3-phosphoglycerate kinase from Corynebacterium glutamicum and its impact on amino acid production

Strain

Specific activity (μmol/min/mg protein)

WT(pEKEx3)

0.9 ± 0.08

WT(pEKEx3-pgk)

2.9 ± 0.2

WT(pVWEx1-pgk)

11.4 ± 0.9

  1. Crude extracts were obtained by sonication of cells cultured in LB medium supplemnted with 1 mM IPTG and 25 μg/mL kanamycin.
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BMC Microbiology

ISSN: 1471-2180

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