Characterization of 3-phosphoglycerate kinase from Corynebacterium glutamicum and its impact on amino acid production

Table 1 Biochemical properties of PGK

Parameter		PGK
Molecular weight		47 kDa
Molecular weight		104 kDa (dimer)
Assay conditions		100 mM TEA-Cl, pH 7.4, 2 mM Mg²⁺, 0.2 mM NADH, 10 U/ml of GAPDH (rabbit muscle), 30°C
Optimal pH		7.0 - 7.4
Optimal temperature		55 - 60°C
Temperature stability		≥60°C
Kinetics
ATP	K_M	0.11 mM
	v_max	150 U/mg
	k_cat	130 s^-1
	k_cat/K_M	592 s^-1 mM^-1
3-PG	K_M	0.26 mM
	v_max	220 U/mg
	k_cat	191 s^-1
	k_cat/K_M	733 s^-1 mM^-1

Values for K_M (mM), V_max (U/mg), and catalytic efficiency (K_cat/K_M = s^-1 mM^-1) were determined for two independent protein purifications.

ISSN: 1471-2180