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Table 3 Effects of homologous sequence length on CAR2 deletion frequency

From: Molecular characterization of KU70 and KU80 homologues and exploitation of a KU70-deficient mutant for improving gene deletion frequency in Rhodosporidium toruloides

Homology length (bp)

Gene deletion frequencya

Improvement (folds)

WT

∆ku70e

50

0 (780)

0 (8)

-

100

0 (620)

21.4% (14)

-

250

0.7% (1668)

30.3% (33)

43.3

500

11.2% (2124)

67.0% (778)

6

750

10.5% (6152)

75.3% (885)

7.2

1000

30.4% (2280)

91.7% (2196)

3

1500

20.5% (2730)

91.0% (4304)

4.4

  1. Note: aNumber in parenthesis indicate number of transformants screened.
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BMC Microbiology

ISSN: 1471-2180

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