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Table 2 Gene deletion frequency in WT and ∆ku70e strains

From: Molecular characterization of KU70 and KU80 homologues and exploitation of a KU70-deficient mutant for improving gene deletion frequency in Rhodosporidium toruloides

Gene target

Homolgy lengtha (bp)

Gene deletion frequencyb

WT

∆ku70e

STE20

800

0 (560)

2.1% (48)

URA3

1000

0 (48)

95.8% (48)

CAR2

750

10.5% (6152)

75.3% (885)

  1. Note: aHomology sequence length on each side of the hygromycin selection cassette; bNumber in parenthesis indicate number of transformants screened.