Characterization of Shiga toxin-producing Escherichia coli isolated from healthy pigs in China

Meng, Qiong; Bai, Xiangning; Zhao, Ailan; Lan, Ruiting; Du, Huamao; Wang, Tao; Shi, Changyou; Yuan, Xuejiao; Bai, Xuemei; Ji, Shaobo; Jin, Dong; Yu, Bo; Wang, Yan; Sun, Hui; Liu, Kai; Xu, Jianguo; Xiong, Yanwen

doi:10.1186/1471-2180-14-5

BMC Microbiology

Table 3 PCR primers used for the detection of STEC virulence or adherence genes

From: Characterization of Shiga toxin-producing Escherichia coli isolated from healthy pigs in China

Targets	Primer	Oligonucleotide sequence (5′-3′)	Amplicon size (bp)	Reference
stx ₁	stx ₁ - F	AAATCGCCATTCGTTGACTACTTCT	370	[61]
	stx ₁-R	TGCCATTCTGGCAACTCGCGATGCA	370	[61]
stx ₂	stx ₂-F	CAGTCGTCACTCACTGGTTTCATCA	283	[61]
	stx ₂-R	GGATATTCTCCCCACTCTGACACC	283	[61]
stx _2e	stx _2e-F	CGGAGTATCGGGGAGAGGC	411	[62]
	stx _2e-R	CTTCCTGACACCTTCACAGTAAAGGT	411	[62]
SLT-II	GK1	ATGAAGTGTATATTATTTAAATGG	1241	[63]
	GK4	TCAGTCATTATTAAACTGCAC	1241	[63]
ehxA	ehxA-F	GGTGCAGCAGAAAAAGTTGTAG	1551	[64]
	ehxA-R	TCTCGCCTGATAGTGTTTGGTA	1551	[64]
hlyA	hlyA 1-F	GACAAAGCACGAAAGATG	2930	[6]
	hlyA 2-R	CAACTGCAATAAAGAAGC	2930	[6]
astA	EAST11a	CCATCAACACAGTATATCCGA	111	[65]
	EAST11b	GGTCGCGAGTGACGGCTTTGT	111	[65]
irp2	irp2- F	AAGGATTCGCTGTTACCGGAC	280	[66]
	irp2- R	TCGTCGGGCAGCGTTTCTTCT	280	[66]
fyuA	fyuA- F	TGATTAACCCCGCGACGGGAA	880	[66]
	fyuA- R	CGCAGTAGGCACGATGTTGTA	880	[66]
eae	eae-F	ACGTTGCAGCATGGGTAACTC	815	[36]
	eae-R	GATCGGCAACAGTTTCACCTG	815	[36]
paa	M155-F1	ATGAGGAAACATAATGGCAGG	350	[67]
	M155-R1	TCTGGTCAGGTCGTCAATAC	350	[67]
iha	iha- F	CAGTTCAGTTTCGCATTCACC	1305	[68]
	iha- R	GTATGGCTCTGATGCGATG	1305	[68]
saa	saa- F	CGTGATGAACAGGCTATTGC	119	[14]
	saa- R	ATGGACATGCCTGTGGCAAC	119	[14]
toxB	toxB- F	ATACCTACCTGCTCTGGATTGA	602	[69]
	toxB- R	TTCTTACCTGATCTGATGCAGC	602	[69]
efa1	efa1- F	GAGACTGCCAGAGAAAG	479	[11]
	efa1- R	GGTATTGTTGCATGTTCAG	479	[11]
lpfA _O157/OI-154	lpfA _O157/OI-154-F	GCAGGTCACCTACAGGCGGC	525	[14]
	lpfA _O157/OI-154-R	CTGCGAGTCGGCGTTAGCTG	525	[14]
lpfA _O157/OI-141	lpfA _O157/OI-141-F	CTGCGCATTGCCGTAAC	412	[70]
	lpfA _O157/OI-141-R	ATTTACAGGCGAGATCGTG	412	[70]
lpfA _O113	lpfA _O113-F	ATGAAGCGTAATATTATAG	573	[9]
	lpfA _O113-R	TTATTTCTTATATTCGAC	573	[9]
F4(K88)	F4-F	GCTGCATCTGCTGCATCTGGTATGG	792	[15]
	F4-R	CCACTGAGTGCTGGTAGTTACAGCC	792	[15]
F5(K99)	F5-F	TGCGACTACCAATGCTTCTG	450	[15]
	F5-R	TATCCACCATTAGACGGAGC	450	[15]
F6(P987)	F6-F	TCTGCTCTTAAAGCTACTGG	333	[15]
	F6-R	AACTCCACCGTTTGTATCAG	333	[15]
F17	F17-F	GGGCTGACAGAGGAGGTGGGGC	411	[15]
	F17-R	CCCGGCGACAACTTCATCACCGG	411	[15]
F18	F18-F	GTGAAAAGACTAGTGTTTATTTC	510	[15]
	F18-R	CTTGTAAGTAACCGCGTAAGC	510	[15]
F41	F41-F	GAGGGACTTTCATCTTTTAG	431	[15]
	F41-R	AGTCCATTCCATTTATAGGC

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ISSN: 1471-2180

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