Table 3 Strains, cells, plasmids and primers used for this study
Strain, cell, plasmid, primer | Relevant description/sequence | Reference or source |
|---|---|---|
Strains | ||
V. cholerae | ||
MO10 pG13 | O139 containing pG13 | This study |
N16961 | Wild type, O1, El Tor, Inaba | Makassar (1971), clinical isolate[8] |
NM06-058 | Wild type, O1, El Tor, Ogawa | Kolkata (1996), clinical isolate |
NM06-058 T283M | Contains a point mutation in gene VC_A0531 on AA position 283 | This study |
RKI-ZBS2-A310-3 | Isolate, O1, El Tor, Inaba | RKI |
RKI-ZBS2-A310-12 | Isolate, O1, El Tor, Ogawa | RKI |
RKI-ZBS2-A198-1 | Isolate, O1, El Tor, Ogawa | RKI |
RKI-ZBS2-A310-25 | Isolate, O139, El Tor | RKI |
RKI-ZBS2-A186-9 | Isolate, O139, El Tor | RKI |
RKI-ZBS2-186-10 | Isolate, O139, El Tor | RKI |
RKI-ZBS2-A220-1 | Isolate, Non O1/O139 | RKI |
RKI-ZBS2-A222-1 | Isolate, Non O1/O139 | RKI |
RKI-ZBS2-A227-1 | Isolate, Non O1/O139 | RKI |
Gram-negative | ||
Acinetobacter baumannii | ATCC 30007 | DSMZ |
E. coli | ESBL, 5044257621-1 | HZI |
E. coli | ETEC | NICED |
E. coli | S17-1 | HZI |
Klebsiella pneumoniae | 50219455 | HZI |
Pseudomonas aeruginosa | 90013687 | HZI |
Salmonella typhimurium | NICED | |
Shigella boydii | NICED | |
Shigella flexneri | NICED | |
Gram-positive | ||
Enterococcus faecalis | ATCC 20212 | HZI |
Staphylococcus aureus | MRSA, N315 | HZI |
Cell line | ||
L929 | Mouse fibroblastic cell line | Derived from commercial source, DSMZ: ACC 2 |
Plasmid | ||
pG13 | Plasmid containing the constitutive expressing G13 promoter- and gfp-gene sequence, ligated in pFPV27 vector, (Kmr) | [9] |
pEX18Ap | Plasmid containing Ampr gene β-lactamase, the sacB gene encoding the levansucrase | HZI |
Oligonucleotide primer | ||
VC_A0531_forw2 | TCACGAACCAACAGGATTAAG | Used for colony PCR and sequencing of the products |
VC_A0531_rev2 | CGGTTAAAGTGGTAGCAGAG | Same as above |
Mut_forw_1 | ACATCATCTAGAGCAGCAGCAACACAAGA (XbaI) | Used for generation of the point mutation |
Mut_rev_1 | ATCGCGCCAAGCGGCA TTTTTAGATCG | Same as above |
Mut_forw_2 | CGATCTAAAAAT GCCGCTTGGCGCGAT | Same as above |
Mut_rev_2 | ACATCAAAGCTTAACATGCGCCACCAGAC (HindIII) | Same as above |
kdpD _del_forw_1 | ACATCATCTAGAGGAATCCATCAAAGAAA (XbaI) | Used for generation of the deletion mutation of kdp D |
kdpD _del_rev_1 | ACAGGATTAAGAAGCAATGAACAGTGAAATTAAGATCCTC | Same as above |
kdpD _del_forw_2 | GAGGATCTTAATTTCACTGTTCATTGCTTCTTAATCCTGT | Same as above |
kdpD _del_rev_2 | ACATCACTGCAGAACACAAGATCCAACAC (PstI) | Same as above |