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Table 3 O157-proteome expressed under anaerobic conditions in dRF and fRF in Experiment I

From: The Escherichia coli O157:H7 bovine rumen fluid proteome reflects adaptive bacterial responses

Protein/Function/Pathway; Name

Accession Number

Molecular Weight (kDa)1

Number of Peptides (Relative Abundance)2

   

dRF

fRF

2,3-bisphosphoglycerate-dependent phosphoglycerate mutase; GpmA

gi|157155502

29 kDa

1 (6)

1 (4)

3-isopropylmalate dehydrogenase/Amino acid Biosynthesis; TtC 3

gi|170684236

40 kDa

1 (6)

1 (4)

Alkyl hydroperoxide reductase protein C/Energy; AhpC

gi|15800320

21 kDa

0

1 (4)

Anaerobic dimethyl sulfoxide reductase/Anaerobic growth/Oxidative Stress; DmsB

gi|145756

23 kDa

1 (6)

1 (4)

Chain A, Crystal Structure Of The Long-Chain Fatty Acid Transporter/Transport; Fadl

gi|203282230

47 kDa

1 (6)

1 (4)

Chain A, Crystal Structure Of Ggbp. Glucose-galactose binding protein/Chemotaxis, Transport; Ggbp 3

gi|126030485

33 kDa

0

1 (4)

Chain A, Structure Of Ivy/ Lysozyme inhibitor; Ivy

gi|29726212

15 kDa

1 (6)

1 (4)

Chaperone protein, stabilizes proteins under heat stress/Heat Stress Related; HchA

gi|15802400

31 kDa

0

1 (4)

Chaperonin, type 1 protein/Protein folding/Transport; GroEL 3

gi|15834378

57 kDa

3 (18)

3 (11)

Cysteine synthase/Amino acid transport and Metabolism; CysK

gi|145686

35 kDa

1 (6)

1 (4)

Cytochrome d ubiquinol oxidase subunit III/Oxidative phosphorylation/Energy; YhCB 3

gi|157148804

15 kDa

0

1 (4)

D-ribose transporter subunit B/Transport; RbsB

gi|110644091

31 kDa

1 (6)

0

DNA-binding transcriptional activator/Osmotically-inducible lipoprotein E; OsmE

gi|15802150

12 kDa

2 (18)

2 (7)

DNA-directed RNA polymerase subunit alpha/Transcription; RpoA

gi|123444073

37 kDa

0

1 (4)

Flagellin/Flagellar assembly/Motility; FliC

gi|15802358

60 kDa

3 (24)

4 (19)

Formate dehydrogenase-O, iron-sulfur subunit, energy metabolism/Anaerobic Respiration, Glyoxylate & Dicarboxylate Metabolism; FdoH

gi|15804482

33 kDa

0

1 (4)

Fructose-bisphosphate aldolase/Glycolysis, Gluconeogenesis, Amino acid Biosynthesis; AldoC

gi|161984958

38 kDa

3 (24)

4 (19)

Glucose-specific PTS system component, phosphorylation/Transport; Crr

gi|15802950

18 kDa

0

1 (4)

Glyceraldehyde 3-Phosphate Dehydrogenase; GadpH 3

gi|1421424

35 kDa

2 (12)

1 (4)

Hypothetical protein CKO_00658/ Uncharacterized; DedA 3

gi|157144929

21 kDa

0

1 (4)

Hypothetical protein EcE24377A_0553/Glyoxylate Utilization; GlxB

gi|157157046

29 kDa

1 (6)

0

Hypothetical protein ECP_2911/Oxidative Stress; YggE

gi|110643066

25 kDa

0

2 (7)

L-asparaginase II, induced by anaerobiosis/Nitrogen and Amino acid Metabolism; AnsB

gi|157157301

37 kDa

0

4 (15)

Maltoporin/Receptor for lambda phage/Transport; LamB 3

gi|110644375

50 kDa

1 (6)

1 (4)

Molecular chaperone/Protein folding/Transport; DnaK

gi|157159481

69 kDa

7 (42)

4 (15)

Molybdopterin biosynthesis protein B/Cofactor Biosynthesis/ Oxidative Stress; MoaB

gi|15800533

19 kDa

0

1 (4)

Outer membrane channel protein, efflux of hydrophobic molecules/Transport; TolC

gi|110643281

54 kDa

0

2 (7)

Outer membrane porin protein C/Tranport of small molecules/Osmotic; OmpC 3

gi|15802768

41 kDa

1 (6)

5 (22)

Outer membrane protein II, porin, receptor, integrity/Membrane Stability; OmpA

gi|146983

26 kDa

2 (12)

3 (11)

Outer membrane protein induced after carbon starvation, stationary phase, environmental stress/ Membrane stability; Slp

gi|110807343

27 kDa

0

1 (4)

Phosphopyruvate hydratase: enolase/Glycolysis, Gluconeogenesis; Eno 3

gi|15832893

46 kDa

0

1 (4)

PTS system, mannose-specific IIAB component/phosphotransferase/Transport; ManX

gi|110641934

35 kDa

0

1 (4)

Putative sulfatase/Inorganic ion transport and metabolism/Transport; YdeN 3

gi|110641672

63 kDa

0

1 (4)

Pyruvate kinase/Glycolysis, Gluconeogenesis, Amino acid Biosynthesis; PykF

gi|110805653

59 kDa

0

1 (4)

Tellurium resistance protein/Stress related; TerD

gi|135596

20 kDa

0

1 (4)

  1. 1KDa, Kilodalton.
  2. 2Relative abundance based on normalized total spectral counts.
  3. 3Proteins not identified in Experiment II (see Table 4).