Skip to main content

Table 2 Toxic activity of P. syringae pv syringae UMAF0158 mutants and mgo operon complemented strains

From: Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA

Strains

E. coli inhibition assay

 

Mangotoxin production

 

PMS

PMS + ornithine

 

Wild type strain and derivative mutants

   

UMAF0158

+

-

Yes

mboA -

-*

-*

No

ΔmgoA

-

-

No

gacA -

-

-

No

gacS -

-

-

No

Transformed with empty vector

   

UMAF0158

+

-

Yes

mboA -

-*

-*

No

ΔmgoA

-

-

No

gacA -

-

-

No

gacS -

-

-

No

Transformed with pLac-mgoBCAD

   

UMAF0158

++

-

Yes

mboA -

-*

-*

No

ΔmgoA

++

-

Yes

gacA -

-

-

No

gacS -

-

-

No

  1. The results are indicated as follows: - absence of inhibition halo, + inhibition halo between 5-10 mm, ++ inhibition halo bigger 10 mm, -* slight toxicity which did not revert in presence of ornithine. Toxic activity, which reverts in the presence of ornithine, denotes the production of mangotoxin.