Figure 3

Phylogeny of the MgoA adenylation domain. Neighbor-joining tree, constructed with MEGA5 using the adenylation domains extracted from nonribosomal peptide synthetases involved in syringomycin, syringopeptin, massetolide A, arthrofactin synthesis and mangotoxin biosynthesis (MgoA). The presence (+) or absence (-) of the mbo operon is shown in the phylogenetic tree. The boxes indicate the different groups of Pseudomonas species which are able to produce mangotoxin when were transformed with pLac-mboABCDEF (mbo operon under its own and P _LAC promoter expression) or pLac-mboFEDCBA (mbo operon under its own promoter expression). Also is indicated the signature sequence of the adenylation domains in each strain. The evolutionary history was inferred using the Neighbor-Joining method [52]. The evolutionary distances were computed using the JTT matrix-based method [53] and are in the units of the number of amino acid substitutions per site. The variation rate among sites was modelled with a gamma distribution. The analysis involved 126 amino acid sequences. There were a total of 328 positions in the final dataset. Evolutionary analyses were conducted in MEGA5 [45]. Bootstrap values (1,000 repetitions) are shown on branches.