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Table 3 qRT-PCR detection of Las from psyllid DNA samples that were collected from different locations in Florida, USA

From: Repertoire of novel sequence signatures for the detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR

Primer pairs

CTvalue of qRT-PCR using infected psyllid DNA samples as template#

Polk

Miami

Highlands

Orange

CREC

P1

32.20

24.70

28.76

26.60

24.87

P2

33.64

25.63

29.96

27.71

25.75

P3

32.19

24.39

29.45

26.57

24.95

P4

33.92

25.47

30.09

28.27

25.81

P5

33.12

24.74

28.54

26.22

25.14

P6

33.52

25.45

29.98

27.80

25.60

P7

32.64

27.29

29.36

27.12

25.42

P8

32.46

24.64

28.82

27.48

25.62

P10

33.20

26.30

30.37

28.65

26.52

P11

34.30

26.47

30.34

28.16

26.14

P16

33.76

24.99

28.97

28.23

26.05

P17

34.87

26.08

30.30

28.45

26.91

P18

34.02

25.40

29.73

28.28

26.38

P23

34.69

25.46

30.43

28.60

26.30

P24

34.84

25.58

30.61

28.71

26.45

P25

33.15

24.10

28.46

26.78

24.77

P26

33.40

25.59

29.74

28.07

25.58

P29

33.42

25.14

29.49

27.73

25.29

P30

36.28

26.53

32.12

29.65

27.07

P31

36.10

27.13

31.67

29.94

27.43

P32

35.53

26.40

31.06

29.22

27.23

P33

33.86

25.01

30.00

27.92

25.65

P34

34.99

25.74

30.93

28.58

26.43

HLBas/r

33.41

25.10

29.09

27.86

25.57

  1. #Las-infected psyllid DNA samples were collected from 5 different locations in Florida, USA. The CT values indicated are average of three replicates for each primer pair.