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Table 1 Primers sequences, annealing temperatures, and expected fragment sizes of PCR reactions targeting specific genes for identification and bacteriocins encoding genes

From: Antagonistic lactic acid bacteria isolated from goat milk and identification of a novel nisin variant Lactococcus lactis

Target

Primers sequences (5'-sequence-3')

Fragment size (bp)

Annealing

Reference

16S rRNA

GCGGCGTGCCTAATACATGC

700

42°C

[29]

 

ATCTACGCATTTCACCGCTAC

   

phe S

CAYCCNGCHCGYGAYATGC

470

46°C

[30]

 

CCWARVCCRAARGCAAARCC

   

lan B

TATGATCGAGAARYAKAWAGATATGG

400-500

40°C

[17, 19]

 

TTATTAIRCAIATGIAYDAWACT

   

lan C

TAATTTAGGATWISYIMAYGG

200-300

40°C

[17, 19]

 

ACCWGKIIIICCRTRRCACCA

   

lan M

ATGCWAGWYWTGCWCATGG

200-300

40°C

[17, 19]

 

CCTAATGAACCRTRRYAYCA

   

nis

GGATAGTATCCATGTCTG

250

55°C

[31]

 

CAATGATTTCGTTCGAAG

   

ent A

CATCATCCATAACTATATTTG

126

56°C

[32]

 

AAATATTATGGAAATGGAGTGTAT

   

ent B

GAAAATGATCACAGAATGCCTA

162

58°C

[32]

 

GTTGCATTTAGAGTATACATTTG

   

ent P

TATGGTAATGGTGTTTATTGTAAT

120

58°C

[32]

 

ATGTCCCATACCTGCCAAAC

   

ent L50AB

STGGGAGCAATCGCAAAATTAG

98

56°C

[32]

 

ATTGCCCATCCTTCTCCAAT

   

ent AS48

GAGGAGTITCATGATTTAAAGA

340

56°C

[32]

 

CATATTGTTAAATTACCAAGCAA