Figure 7
Heatmap of RNA Sequencing comparing JKD6159 ( aryK inactive) to JKD6159_AraC r ( aryK intact). RNA seq was performed in duplicate from stationary phase cultures. This heatmap, clustered on expression profiles, was created based on log2 transformed counts to identify consistent changes in expression profiles between strains. To be included in the heat map, genes were required to have at least 1000 counts (reads), totaled over all samples, where the standard deviation of log2 expression differences had to exceed two. The heatmap highlights significant aryK-dependent changes, in particular genes involved in the regulation of central metabolic functions.