Table 1 Deduced functions of ORFs in the biosynthetic gene cluster of PLYA
Gene | Sizea | Accession no. | Proposed function | Homologous protein species | Identity/Similarity |
|---|---|---|---|---|---|
orf03399 | 384 | YP_003099796 | Nucleotidyl transferase | Actinosynnema mirum DSM 43827 | 64/73 |
orf03396 | 309 | YP_004903951 | putative sugar kinase | Kitasatospora setae KM-6054 | 50/62 |
orf1 | 422 | YP_003099794 | 3-dehydroquinate synthase | Actinosynnema mirum DSM 43827 | 56/69 |
orf2 | 128 | EID72461 | MarR family transcriptional regulator | Rhodococcus imtechensis RKJ300 | 71/83 |
orf3 | 146 | ZP_09957194 | Hypothetical protein | Streptomyces chartreusis NRRL 12338 | 75/84 |
orf4 | 566 | CAJ61212 | Putative polyketide oxygenase/hydroxylase | Frankia alni ACN14a | 77/83 |
orf5 | 377 | ZP_04706918 | Alcohol dehydrogenase BadC | Streptomyces roseosporus NRRL 11379 | 76/86 |
orf6 | 312 | ZP_06582592 | 3-oxoacyl-[acyl-carrier-protein] synthase III | Streptomyces roseosporus NRRL 15998 | 71/82 |
orf7 | 82 | ZP_04706920 | Hypothetical protein | Streptomyces roseosporus NRRL 11379 | 59/75 |
orf8 | 82 | ZP_04706921 | Dihydrolipoamide succinyltransferase | Streptomyces roseosporus NRRL 11379 | 65/81 |
orf9 | 326 | ZP_06582595 | 2-oxoisovalerate dehydrogenase | Streptomyces roseosporus NRRL 15998 | 75/87 |
orf10 | 303 | ZP_04706923 | Pyruvate dehydrogenase | Streptomyces roseosporus NRRL 11379 | 74/84 |
plyA | 71 | YP_640626 | MbtH-like protein | Mycobacterium sp. MCS | 80/87 |
plyB | 225 | YP_712760 | Putative regulator | Frankia alni ACN14a | 76/84 |
plyC | 528 | YP_712761 | A | Frankia alni ACN14a | 77/85 |
plyD | 77 | YP_712762 | PCP | Frankia alni ACN14a | 85/94 |
plyE | 395 | YP_712763 | Putative hydroxylase | Frankia alni ACN14a | 76/86 |
plyF | 2583 | ABV56588 | C-A-PCP-E-C-A-PCP | Kutzneria sp. 744 | 56/68 |
plyG | 2809 | ZP_05519638 | C-A-PCP-E-C-A-PCP | Streptomyces hygroscopicus ATCC 53653 | 73/82 |
plyH | 1662 | BAH04161 | C-A-M-PCP-TE | Streptomyces triostinicus | 72/82 |
plyI | 247 | YP_712767 | TE | Frankia alni ACN14a | 80/87 |
plyJ | 312 | YP_003112824 | Daunorubicin resistance ABC transporter | Catenulispora acidiphila DSM 44928 | 78/90 |
plyK | 253 | YP_712769 | ABC transporter system | Frankia alni ACN14a | 71/81 |
plyL | 1043 | YP_003112826 | Transcriptional regulator | Catenulispora acidiphila DSM 44928 | 72/80 |
plyM | 412 | AAT45271 | Cytochrome P450 monooxygenase | Streptomyces tubercidicus | 43/59 |
plyN | 450 | ZP_04604097 | Aminotransferase class I and II | Micromonospora sp. ATCC 39149 | 58/70 |
plyO | 308 | ZP_03862696 | Polyketide fumonisin | Kribbella flavida DSM 17836 | 48/64 |
plyP | 270 | ZP_04292518 | L-proline 3-hydroxylase type II | Bacillus cereus R309803 | 32/51 |
plyQ | 88 | YP_712776 | PCP | Frankia alni ACN14a | 76/89 |
plyR | 415 | YP_712777 | Cytochrome P450 monooxygenase | Frankia alni ACN14a | 77/85 |
plyS | 245 | AAT45287 | TE | Streptomyces tubercidicus | 70/81 |
plyT | 1031 | YP_712779 | KS-AT-ACP | Frankia alni ACN14a | 71/80 |
plyU | 1872 | YP_712780 | KS-AT-DH-KR-ACP | Frankia alni ACN14a | 69/79 |
plyV | 2199 | YP_712781 | KS-AT-DH-ER-KR-ACP | Frankia alni ACN14a | 70/78 |
plyW | 1041 | YP_712782 | KS-AT-ACP | Frankia alni ACN14a | 72/82 |
plyX | 1080 | YP_712783 | C-A-PCP | Frankia alni ACN14a | 62/72 |
plyY | 253 | ZP_04472110 | TE | Streptosporangium roseum DSM 43021 | 70/78 |
plyZ | 79 | YP_001612061 | LysR family transcriptional regulator | Sorangium cellulosum | 65/77 |
orf11 | 287 | YP_702564 | Hypothetical protein | Rhodococcus jostii RHA1 | 56/73 |
orf14742 | 170 | YP_002777514 | Hypothetical protein ROP_03220 | Rhodococcus opacus B4 | 51/63 |