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Table 2 Generation time (minutes) of Escherichia coli strains in different culture media*

From: New function for Escherichia coli xanthosine phophorylase (xapA): genetic and biochemical evidences on its participation in NAD+ salvage from nicotinamide

No.

Strain

Pathway Deficiency

Medium M9

M9 + NA

M9 + NAD+

M9 + NAM

Expected

Observed

Expected

Observed

Expected

Observed

Expected

Observed

1

BW25113

None

+

65.8

+

49.8

+

50.5

+

49.4

2

ΔnadC

dn

–

–

+

49.4

+

49.4

+

53.4

3

ΔnadC ΔpncA

dn, I

–

–

+

50.3

+

49.2

–

380.8

4

ΔnadC ΔpncA ΔxapA

dn, I

–

–

+

49.2

+

50.0

–

620.4

5

ΔnadC ΔpncA ΔxapA/pBAD-xapA

dn, I

–

NT

+

NT

+

+

–

376.4

6

ΔnadC ΔpncA ΔxapA/pBAD-EGFP

dn, I

–

NT

+

NT

+

+

–

626.8

7

ΔnadC ΔpncA ΔnadR

dn, I, III

–

–

+

51.1

+

NT

–

–

8

ΔnadC ΔpncA ΔxapA ΔnadR

dn, I, III

–

–

+

49.7

+

NT

–

–

  1. *Notes: NA, nicotinic acid; NAM, nicotinamide; NAD+, nicotinamide adenine dinucleotide; NT, not tested; –, No proliferation; +, proliferation; dn, de novo NAD+ synthesis; I, NAD+ salvage pathway I; III, NAD+ salvage pathway III.