Skip to main content

Table 2 Generation time (minutes) of Escherichia coli strains in different culture media*

From: New function for Escherichia coli xanthosine phophorylase (xapA): genetic and biochemical evidences on its participation in NAD+ salvage from nicotinamide

No. Strain Pathway Deficiency Medium M9 M9 + NA M9 + NAD+ M9 + NAM
Expected Observed Expected Observed Expected Observed Expected Observed
1 BW25113 None + 65.8 + 49.8 + 50.5 + 49.4
2 ΔnadC dn + 49.4 + 49.4 + 53.4
3 ΔnadC ΔpncA dn, I + 50.3 + 49.2 380.8
4 ΔnadC ΔpncA ΔxapA dn, I + 49.2 + 50.0 620.4
5 ΔnadC ΔpncA ΔxapA/pBAD-xapA dn, I NT + NT + + 376.4
6 ΔnadC ΔpncA ΔxapA/pBAD-EGFP dn, I NT + NT + + 626.8
7 ΔnadC ΔpncA ΔnadR dn, I, III + 51.1 + NT
8 ΔnadC ΔpncA ΔxapA ΔnadR dn, I, III + 49.7 + NT
  1. *Notes: NA, nicotinic acid; NAM, nicotinamide; NAD+, nicotinamide adenine dinucleotide; NT, not tested; –, No proliferation; +, proliferation; dn, de novo NAD+ synthesis; I, NAD+ salvage pathway I; III, NAD+ salvage pathway III.