Figure 3

Dose-dependent effects of NAD ⁺ on the growth of Escherichia coli mutant with triple-deletion (BW25113Δ nadC Δ pncA Δ xapA ). A) Growth curve of the mutant in M9 minimal medium supplied with various concentration of NAD⁺. B) The relationship of the inverse of the NAD⁺ concentration (from 0.1 to 1 μg/ml) to the bacterial generation time in M9/NAD⁺ medium for 7 h. C) The relationship of the NAD⁺ concentration (from 0.1 to 1 μg/ml) to the OD₆₀₀ of the mutant grown in M9/NAD⁺ medium for 7 h.