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Table 3 Transcript-derived fragments (TDFs) from L. rhamnosus PR1019 over-expressed in CB compared to MRS

From: Transcriptomic clues to understand the growth of Lactobacillus rhamnosus in cheese

TDF no

Primer combination

Length (bp)

Biological functiona

Organism annotationb

Max identity - E-valuec

Accession no.

Pathway assignmentd

COGe

KEGG

37

AC/AT

396

Guanylate kinase (EC 2.7.4.8)

L. rhamnosus GG

98% - 1e-84

YP_003171760.1

COG0194 [F]

ko00230: Purine metabolism

40

AC/AT

302

Putative phosphoketolase (EC 4.1.2.9)

L. rhamnosus GG

99% - 3e-57

YP_005864692.1

COG3957 [G]

ko00030: Pentose phosphate pathway

48

AC/AT

199

Monooxygenase

L. rhamnosus Lc 705

95% - 6e-17

YP_003174467.1

COG2329: Conserved protein involved in polyketide biosynthesis related to monooxygenase [R]

_

54

AC/AT

137

Hypothetical protein

L. rhamnosus

77% - 2e-07

WP_005689523.1

_

_

72

AC/AT

340

Lipoteichoic acid synthase LtaS Type IIa (EC 3.1.6)

L. rhamnosus Lc 705

100% - 5e-43

YP_003173514.1

COG1368: Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily [M]

_

76

AC/AT

433

Conserved hypothetical protein

L. rhamnosus Lc 705

85% - 9e-27

YP_003174890.1

_

_

86

AC/AT

109

L-xylulose 5-phosphate 3-epimerase (EC 5.1.3.22)

L. rhamnosus GG

94% - 9e-13

YP_003172471.1

COG3623 [G]

ko00040: Pentose and glucuronate interconversions

93

AC/AT

305

Pyruvate oxidase (EC 1.2.3.3)

L. rhamnosus GG

93% - 5e-40

YP_003171582.1

COG3961: Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes [G]

ko00620: Pyruvate metabolism

95

AC/AT

229

Plasmid pNCD0151

L. casei

98% - 4e-68

Z50861.1

_

_

97

AC/AT

227

Plasmid pNCD0151

L. casei

96% - 3e-64

Z50861.1

_

_

106

AC/AT

170

Plasmid pNCD0151

L. casei

97% - 9e-48

Z50861.1

_

_

120

AC/AT

107

Hypothetical protein

L. casei

96% - 4e-10

WP_003574536.1

_

_

121

AC/AT

105

Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)

L. rhamnosus Lc 705

92% - 5e-08

YP_003174148.1

COG0131 [E]

ko00340: Hystidine metabolism

122

AC/AT

102

Plasmid pNCD0151

L. casei

96% - 5e-23

Z50861.1

_

_

162

AT/AC

350

Calcineurin-like phosphoesterase family protein

L. rhamnosus ATCC 8530

97% - 3e-70

YP_005872999.1

COG0737: 5′-nucleotidase/2′,3′-cyclic phosphodiesterase and related esterases [F]

_

168

AT/AC

238

Plasmid pNCD0151

L. casei

98% - 1e-68

Z50861.1

_

_

170

AT/AC

222

Hypothetical protein

L. rhamnosus GG

98% - 5e-34

YP_003171844.1

_

_

211

AT/AT

240

5S ribosomal RNA

L. rhamnosus GG

98% - 2e-11

NR_103302.1

_

_

212

AT/AT

234

5S ribosomal RNA

L. rhamnosus GG

98% - 4e-09

NR_103302.1

_

_

  1. aWhen available, EC numbers assigned to the putative enzymatic reactions are provided.
  2. bThe column indicates the microorganism of the best hit from BLASTX search.
  3. cMax identity and E-value from the best hit of BLASTX search are provided.
  4. dPathway assignment was performed according to COG functional categories and KEGG pathway database.
  5. eE, Amino acid transport and metabolism; F, Nucleotide transport and metabolism; G, Carbohydrate transport and metabolism; M, Cell wall/membrane/envelope biogenesis; R, General function prediction only.