Transcriptomic clues to understand the growth of Lactobacillus rhamnosus in cheese

BMC Microbiology

Table 2 qPCR settings and relative transcript abundance

Target gene	Primer sequence (5′-3′)^a	Product size (bp)	PCR efficiency	Expression ratio^b
spxB	fwd: TACCGGAAACTGCTTGGTATC	155	1.93	8.97
spxB	rev: CTGGAAAACCGCATCTTTGT	155	1.93	8.97
ulaE	fwd: CACTAGCCAAATCAATCGCC	90	2.05	5.78
ulaE	rev: GCCATCGTCGGTTTCCATTA	90	2.05	5.78
xfp	fwd: CGTGAAGAAGGCGATATC	215	2.01	5.98
xfp	rev: TTCCAAGTCCACTCCTGA	215	2.01	5.98
16S rDNA	fwd: GCYTAACACATGCAAGTCGA	500	1.85	/
	rev: GTATTACCGCGGCTGCTGG

^aPrimer sets were designed based on the sequences of cDNA-AFLP fragments. Primers for 16S rDNA gene were designed as reported by Giraffa et al. [24].
^bTarget gene expression was calculated relative to 16S rDNA as a reference gene using the efficiency-corrected ΔΔC _T method [23]. The relative expression ratios in CB compared to MRS are shown.

ISSN: 1471-2180