Table 1 Primer and adaptor sequences
From: Transcriptomic clues to understand the growth of Lactobacillus rhamnosus in cheese
Primer/adaptor | Sequence (5′-3′) | Application |
|---|---|---|
Adaptor EcoRI-f | CTCGTAGACTGCGTACC | Ligation |
Adaptor EcoRI-r | AATTGGTACGCAGTCTAC | Ligation |
Adaptor MseI-f | GACGATGAGTCCTGAG | Ligation |
Adaptor MseI-r | TACTCAGGACTCAT | Ligation |
EcoRI-0 | GACTGCGTACCAATTC | Non-selective PCR |
MseI-0 | GATGAGTCCTGAGTAA | Non-selective PCR |
5′DY-EcoRI-AT | GACTGCGTACCAATTCAT | Selective PCR |
5′DY-EcoRI-AC | GACTGCGTACCAATTCAC | Selective PCR |
MseI-AT | GATGAGTCCTGAGTAAAT | Selective PCR |
MseI-AC | GATGAGTCCTGAGTAAAC | Selective PCR |
EcoRI-AC | GACTGCGTACCAATTCAC | Re-amplification PCR |
EcoRI-AT | GACTGCGTACCAATTCAT | Re-amplification PCR |