Figure 9From: Pseudomonas pyocyanin stimulates IL-8 expression through MAPK and NF-κB pathways in differentiated U937 cells Effects of MAPK inhibitors on PCN-induced NF- κB signaling pathway. U937 cells were stimulated with PCN at 50 μM for the time periods indicated with or without pretreatment by MAPK and NF-κB inhibitors: SB 203580 (50 μM), PD98059 (50 μM) and PDTC (200 μM) for 1 h. Cell proteins were then collected and NF-κB p65 protein expression was detected with Western blotting.Back to article page