Figure 4

NF-κB inhibitor reduces PCN-induced IL-8 release. Different concentrations of NF-κB blocker (PDTC at 50, 100, or 200 μmol/L) were added into fresh medium of PMA-differentiated U937 cells 60 min before PCN was added. Cells were exposed to a fixed concentration of PCN (50 μM) for 24 h. Supernatants were harvested for measuring IL-8 by ELISA. *p < 0.05, **p < 0.01 compared with the PCN group. PMA: phorbol 12-myristate 13-acetate.