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Figure 2 | BMC Microbiology

Figure 2

From: Pseudomonas pyocyanin stimulates IL-8 expression through MAPK and NF-κB pathways in differentiated U937 cells

Figure 2

PCN increases IL-8 release in PMA-differentiated U937 cells. (A) Different concentrations of PCN (5 μM, 25 μM, or 50 μM) were added to the cell cultures for 24 h. Supernatants were harvested for measuring IL-8 secretion by ELISA. (B) A fixed concentration of PCN (50 μM) was added to the cell cultures for 8, 16 or 24 h. Supernatants were harvested for measuring IL-8 level by ELISA. Values represented are the mean ± SD of four independent experiments in triplicate. **p < 0.01 compared with PMA-differentiated U937 cells. PMA: phorbol 12-myristate 13-acetate.

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