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Table 2 PAO1 growth-impairing inserts including loci belonging to a single operon

From: A shotgun antisense approach to the identification of novel essential genes in Pseudomonas aeruginosa

Insert namea

Operon loci b

Gene name and product annotationc

Function classc

Species containing orthologs in DEGd

E6

PA1037

yicG - conserved hypothetical protein (4)

Hypothetical, unclassified, unknown

 

PA1038

hypothetical protein (4)

 

PA1039

ychJ - hypotetical protein (4)

 

PA1040

hypothetical protein (4)

 

S9B6a

PA1089

conserved hypothetical protein (4)

Hypothetical, unclassified, unknown

 

PA1090

conserved hypothetical protein (4)

 

PA1088

hypothetical protein (4)

 

S9B6b

PA0393

proC - pyrroline-5-carboxylate reductase (1)

Amino acid biosynthesis and metabolism

E. coli, M. tuberculosis, A. baylyi

PA0392

yggT - conserved hypothetical protein (4)

Hypothetical, unclassified, unknown

 

PA0394

yggS - conserved hypothetical protein (4)

 

S2A4

PA1001 e

phnA - anthranilate synthase component I (1)

Adaptation, protection; amino acid biosynthesis

 

PA1002 e

phnB - anthranilate synthase component II (1)

 
  1. aInserts with antisense orientation are in bold.
  2. bLoci included in the insert are in bold.
  3. cAnnotations according to the Pseudomonas Genome Database (http://www.pseudomonas.com) [27]. Numbers inside parenthesis indicate the classes of product name confidence. Class1: Function experimentally demonstrated in P. aeruginosa; Class 2: Function of highly similar gene experimentally demonstrated in another organism; Class 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene. Class 4: Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences.
  4. dDEG: Database of Essential Genes (DEG 7.0) (http://www.essentialgene.org) [20].
  5. ePrevious reports [34, 35] did not mention growth defects associated to deletion of phnAB genes.