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Table 1 Strain and plasmid list

From: The Vibrio cholerae diguanylate cyclase VCA0965 has an AGDEF active site and synthesizes cyclic di-GMP

Strain or plasmid

Relevant features

Source or citation

Strain

  

Vibrio cholerae

  

C6706str2

Wild type strain

[29]

CW2034

ΔvpsL

[32]

Plasmid

  

pEVS141

 

[30]

pCMW75

pEVS143 backbone, overexpression of QrgB (Vibrio harveyi DGC) under Ptac promoter

[31]

pVCA0965

pEVS143 backbone, overexpression of VCA0965 under Ptac promoter

[30]

pVCA0965-A Site

pEVS143 backbone, overexpression of VCA0965 E287A under Ptac promoter

This study

pVCA0965-I Site

pEVS143 backbone, overexpression of VCA0965 R267A, D270A under Ptac promoter

This study

pVCA0965-AI Site

pEVS143 backbone, overexpression of VCA0965 R267A, D270A, E287A under Ptac promoter

This study

pVCA0965(180–396)

pET28b backbone, overexpression of VCA0965 (180–396) with a 6 histidine C terminal tag

This study