Figure 7
SDS-PAGE analysis of L. amylovorus strains. Intact cells of L. amylovorus DSM 16698 (lane 1), DSM 20531T (lane 2), GRL 1112 (lane 3), GRL 1114 (lane 4), GRL 1115 (lane 5), GRL 1116 (lane 6), GRL 1117 (lane 7) and GRL 1118 (lane 8) from 50 μl of overnight cultures (A600nm = 6.4) were boiled in standard Laemmli sample buffer (extracting surface proteins) and the supernatants were analyzed by standard SDS-PAGE in a 12% gel. Arrowheads indicate SlpA (44 kDa), SlpB (50 kDa) and SlpC (40 kDa) of L. amylovorus DSM 16698 (lane 1) and SlpA (61 kDa) and B (49 kDa) of GRL 1117 (lane 7).