TY - JOUR AU - Hynönen, Ulla AU - Kant, Ravi AU - Lähteinen, Tanja AU - Pietilä, Taija E. AU - Beganović, Jasna AU - Smidt, Hauke AU - Uroić, Ksenija AU - Åvall-Jääskeläinen, Silja AU - Palva, Airi PY - 2014 DA - 2014/07/28 TI - Functional characterization of probiotic surface layer protein-carrying Lactobacillus amylovorus strains JO - BMC Microbiology SP - 199 VL - 14 IS - 1 AB - Adhesiveness to intestinal epithelium, beneficial immunomodulating effects and the production of pathogen-inhibitory compounds are generally considered as beneficial characteristics of probiotic organisms. We showed the potential health-promoting properties and the mechanisms of probiotic action of seven swine intestinal Lactobacillus amylovorus isolates plus the type strain (DSM 20531T) by investigating their adherence to porcine intestinal epithelial cells (IPEC-1) and mucus as well as the capacities of the strains to i) inhibit the adherence of Escherichia coli to IPEC-1 cells, ii) to produce soluble inhibitors against intestinal pathogens and iii) to induce immune signaling in dendritic cells (DCs). Moreover, the role of the L. amylovorus surface (S) –layers - symmetric, porous arrays of identical protein subunits present as the outermost layer of the cell envelope - in adherence to IPEC-1 cells was assessed using a novel approach which utilized purified cell wall fragments of the strains as carriers for the recombinantly produced S-layer proteins. SN - 1471-2180 UR - https://doi.org/10.1186/1471-2180-14-199 DO - 10.1186/1471-2180-14-199 ID - Hynönen2014 ER -