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Table 1 Media used in the screening panel

From: The Unculturables: targeted isolation of bacterial species associated with canine periodontal health or disease from dental plaque

Media Target bacteria
Columbia blood agar (CBA) (Oxoid, UK) Aerobes or Anaerobes when supplemented with H + M
Fastidious anaerobe agar (FAA) + H + M (LabM, UK) Anaerobes
Tryptone soya agar (TSA) (Oxoid, UK) Aerobes or Anaerobes when supplemented with H + M
Heart infusion agar (HIA) (Oxoid, UK) Anaerobes
Artificial saliva agar (ASA) (see text) + H + M Anaerobes
Blood agar base no. 2 (Oxoid, UK) Aerobes or Anaerobes when supplemented with H + M
All of the above + kanamycin (75 μg/ml; Sigma-Aldrich, UK) Gram-negative bacteria
All of the above + Phenylethyl alcohol (PEA) (0.25% vol: final vol; Sigma-Aldrich, UK) Gram-positive cocci
  1. All media were supplemented with 5% horse blood. H + M = hemin (5 μg/ml; Sigma-Aldrich, UK) and menadione (0.5 μg/ml; Sigma-Aldrich, UK).
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BMC Microbiology

ISSN: 1471-2180

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