Skip to main content

Table 1 Media used in the screening panel

From: The Unculturables: targeted isolation of bacterial species associated with canine periodontal health or disease from dental plaque

Media

Target bacteria

Columbia blood agar (CBA) (Oxoid, UK)

Aerobes or Anaerobes when supplemented with H + M

Fastidious anaerobe agar (FAA) + H + M (LabM, UK)

Anaerobes

Tryptone soya agar (TSA) (Oxoid, UK)

Aerobes or Anaerobes when supplemented with H + M

Heart infusion agar (HIA) (Oxoid, UK)

Anaerobes

Artificial saliva agar (ASA) (see text) + H + M

Anaerobes

Blood agar base no. 2 (Oxoid, UK)

Aerobes or Anaerobes when supplemented with H + M

All of the above + kanamycin (75 μg/ml; Sigma-Aldrich, UK)

Gram-negative bacteria

All of the above + Phenylethyl alcohol (PEA) (0.25% vol: final vol; Sigma-Aldrich, UK)

Gram-positive cocci

  1. All media were supplemented with 5% horse blood. H + M = hemin (5 μg/ml; Sigma-Aldrich, UK) and menadione (0.5 μg/ml; Sigma-Aldrich, UK).