Figure 3

LPS and gingipains are released from P. gingivalis . (A). The release of LPS from THP-1 cells stimulated with P. gingivalis (MOI 100). Significant accumulation of LPS was observed in culture supernatants of THP-1 triggered by W50, W50HK and K1A in relation to the negative control. (B). Relative concentration of argine and lysine gingipains present in the culture supernatants after 24 hour stimulation of THP-1 cells with MOI 100 viable ATCC33277 (ATCC), W50, E8 or K1A (N.D: not detected). The differnces between the different stimulations were analyzed by one-way ANOVA. Values represent the mean ± SD. (*p < 0.05; **p < 0.01; ***p < 0.001; n = 3).