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Table 2 Polyamine profile in Caco-2 cells after 6 h of exposure to viable L.GG (10 8CFU/ml), L.GG-HK and L.GG-CM, alone or in combination with gliadin (1 mg/ml)

From: Lactobacillus GG restoration of the gliadin induced epithelial barrier disruption: the role of cellular polyamines

 

Control

Viable L.GG

L.GG-HK

L.GG-CM

Gliadin

Gliadin + Viable L.GG

Gliadin + L.GG-HK

Gliadin + L.GG-CM

Putrescine

0.15 ± 0.1a

0.12 ± 0.1a

0.1 ± 0.2a

0.12 ± 0.1a

0.2 ± 0.005a

0.2 ± 0.008a

0.16 ± 0.005a

0.2 ± 0.01a

Spermidine

6.9 ± 0.08a

3.3 ± 0.1c

3.8 ± 0.2c

6.8 ± 0.09a

9.3 ± 0.05b

6.0 ± 0.06a

7.1 ± 0.05a

8.2 ± 0.2ab

Spermine

7.8 ± 0.05a

4.3 ± 0.04c

5.3 ± 0.5c

7.5 ± 0.05a

11.1 ± 0.3b

4.3 ± 0.1c

8.9 ± 0.03a

11.3 ± 0.09 ab

Total polyamines

14.3 ± 0.3a

7.9 ± 0.5c

9.1 ± 0.6c

14.4 ± 0.5a

20.9 ± 0.8b

10.3 ± 0.4c

15.9 ± 0.3a

20.01 ± 0.5b

  1. All data represent the results of three different experiments (mean ± SEM). For each treatment mean values not sharing a common superscript differ significantly (P < 0.05, Kruskal-Wallis analysis of variance and Dunn’s Multiple Comparison Test). Polyamines are in nmol/mg protein.