Skip to main content

Table 1 Strains used in this study

From: Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast

No. Genotype Figures
NT4 h 90 spo5(S365P)-GFP-kan ade6-M216 leu1 ura4 Figure 1D, Figure 4AB and Additional file 3: Figure S3BC
NT92 h 90 spo5(F341L)-GFP-kan ade6-M216 leu1 ura4 Figure 1D, Figure 4B and Additional file 3: Figure S3BC
NT180 h 90 spo5-GFP-kan mei2-mCherry-hph ade6-M216 leu1 Figure 2D
NT184 h 90 spo5-GFP-kan ade6-M216 leu1 ura4 Figure 1BD, Figure 3A-CE and Additional file 3: Figure S3BC
NT196 h 90 spo5(S365P)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1C
NT205 h 90 spo5(1–296)-GFP-kan ade6-M216 leu1 ura4 Figure 1B and Additional file 1: Figure S1
NT207 h 90 spo5(1–366)-GFP-kan ade6-M216 leu1 ura4 Figure 1B
NT209 h 90 spo5(1–456)-GFP-kan ade6-M216 leu1 ura4 Figure 1B
NT403 h 90 spo5-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1AC, Figure 2A and Figure 3D,
   Additional file 1: Figure S1, Additional file 2: Figure S2 and Additional file 3: Figure S3A
NT648 h 90 spo5(1–525)-GFP-kan ade6-M216 leu1 ura4 Figure 1B
NT716 h 90 spo5(1–525)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1A
NT745 h 90 spo5(F341L)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1C
NT798 h 90 spo5(1–296)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1A
NT799 h 90 spo5(1–366)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1A
NT814 h 90 spo5(1–456)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1A
NT973 h 90 spo5(RRM1∆ , ∆ 297-366)-GFP-kan ade6-M216 leu1 ura4 Figure 1B and Figure 3E
NT974 h 90 spo5(RRM2∆ , ∆ 385-456)-GFP-kan ade6-M216 leu1 ura4 Figure 1B and Additional file 1: Figure S1
NT1001 h 90 spo5(RRM1∆ , ∆ 297-366)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1A and Figure 3D
NT1002 h 90 spo5(RRM2∆ , ∆ 385-456)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1A
NT1168 h +/ h - spo5-GFP-kan/ spo5-GFP-kan ade6-M216/ade6-M210 leu1/leu1 ura4/ura4 Figure 2BC and Figure 5B
NT1208 h 90 spo5(S365P)-GFP-kan rae1-167 ade6-M216 leu1 ura4 Additional file 1: Figure S1
NT1495 h 90 spo5::ura4 + ::spo5(F427A)-GFP-kan ade6-M216 leu1 ura4 Figure 1D
NT1568 h 90 spo5::ura4 + ade6-M216 leu1 ura4 Figure 4BC and Additional file 3: Figure S3BC
NT1622 h 90 spo5::ura4 + ::spo5(F427A)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1C
NT1632 h 90 spo5::ura4 + ::spo5(F341A)-GFP-kan ade6-M216 leu1 ura4 Figure 1D
NT1634 h 90 spo5::ura4 + ::spo5(F341A, F427A)-GFP-kan ade6-M216 leu1 ura4 Figure 1D and Additional file 1: Figure S1
NT1641 h 90 spo5::ura4 + ::spo5(F341A)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1C
NT1648 h 90 spo5::ura4 + ::spo5(F341A, F427A)-GFP-kan cut11-4mRFP-hph CFP-atb2-nat ade6-M216 leu1 ura4 Figure 1C and Additional file 2: Figure S2
NT1677 h 90 spo5-GFP-kan rae1-167 ade6-M216 leu1 ura4 Additional file 1: Figure S1
NT1743 h 90 spo5 + NLS-GFP-kan ade6-M216 leu1 ura4 Figure 3A-C and Additional file 1: Figure S1
NT1769 h 90 spo5-GFP-kan cut11-4mRFP-hph rae1-167 ade6-M216 leu1 Figure 2A and Additional file 3: Figure S3A
NT1793 h 90 pabp-GFP-kan spo5::ura4 + cut11-4mRFP-hph ade6-M216 leu1 ura4 Figure 2E
NT1898 h 90 spo5(S365P)-GFP-kan rae1-167 cut11-4mRFP-hph ade6-M216 leu1 ura4 Additional file 3: Figure S3A
NT1905 h 90 pabp-GFP-kan rae1-167 cut11-4mRFP-hph ade6-M216 leu1 ura4 Figure 2E
NT1922 h 90 spo5(RRM1∆ ) + NES-GFP ade6-M216 leu1 ura4 Figure 3E
NT1943 h 90 spo5(RRM1∆ ) + NES-GFP cut11-4mRFP-hph ade6-M216 leu1 ura4 Figure 3D
NT2130 h +/ h - ade6-M216/ade6-M210 leu1/leu1 ura4/ura4 Additional file 4: Figure S4A-C
NT2132 h +/ h - spo5::ura4 + /spo5::ura4 + ade6-M216/ade6-M210 leu1/leu1 ura4/ura4 Additional file 4: Figure S4A-C
  1. The original rae1-167 strain was provided by Ravi Dhar [25].
Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us