Multiresistant extended-spectrum β-lactamase-producing Enterobacteriaceae from humans, companion animals and horses in central Hesse, Germany

Table 1 PCR primers used to detect β-lactamase genes, PMQR genes and E. coli phylogenetic groups

Primer	Sequence (5’ - 3’)	Target	Size of product (bp)	Reference
TEM_f	ATGAGTATTCAACATTTCCG	bla _TEM	851	[11]
TEM_r	TTAATCAGTGAGGCACCTAT	bla _TEM	851	[11]
SHV_f	GCAAAACGCCGGGTTATTC	bla _SHV	940	[11]
SHV_r	GGTTAGCGTTGCCAGTGCT	bla _SHV	940	[11]
CTX-M_f	TCTTCCAGAATAAGGAATCCC	bla _CTX-M	909	[12]
CTX-M_r	CCGTTTCCGCTATTACAAAC	bla _CTX-M	909	[12]
OXA-1_f	AGCAGCGCCAGTGCATCA	bla _OXA-1	700	[13]
OXA-1_r	ATTCGACCCCAAGTTTCC	bla _OXA-1	700	[13]
OXA-48_f	AAATCACAGGGCGTAGTTGTG	bla _OXA-48	555	[11]
OXA-48_r	GACCCACCAGCCAATCTTAG	bla _OXA-48	555	[11]
qnrA_f	AGAGGATTTCTCACGCCAGG	qnrA	619	[14]
qnrA_r	GCAGCACTATKACTCCCAAGG	qnrA	619	[14]
qnrB_f	GGMATHGAAATTCGCCACTG	qnrB	264	[16]
qnrB_r	TTTGCYGYYCGCCAGTCGAA	qnrB	264	[16]
qnrC_f	GGGTTGTACATTTATTGAATC	qnrC	447	[17]
qnrC_r	TCCACTTTACGAGGTTCT	qnrC	447	[17]
qnrD_f	CGAGATCAATTTACGGGGAATA	qnrD	582	[18]
qnrD_r	AACAAGCTGAAGCGCCTG	qnrD	582	[18]
qnrS_f	GCAAGTTCATTGAACAGGCT	qnrS	428	[16]
qnrS_r	TCTAAACCGTCGAGTTCGGCG	qnrS	428	[16]
qepA_f	CTGCAGGTACTGCGTCATG	qepA	403	[15]
qepA_r	CGTGTTGCTGGAGTTCTTC	qepA	403	[15]
aac_f	TTGCGATGCTCTATGAGTGGCTA	aac(6’)-Ib	482	[19]
aac_r	CTCGAATGCCTGGCGTGTTT	aac(6’)-Ib	482	[19]
chuA.1	GACGAACCAACGGTCAGGAT	chuA	279	[21]
chuA.2	TGCCGCCAGTACCAAAGACA	chuA	279	[21]
YjaA.1	TGAAGTGTCAGGAGACGCTG	yajA	211	[21]
YjaA.2	ATGGAGAATGCGTTCCTCAAC	yajA	211	[21]
TspE4C2.1	GAGTAATGTCGGGGCATTCA	TspE4C2	152	[21]
TspE4C2.2	CGCGCCAACAAAGTATTACG	TspE4C2	152	[21]

ISSN: 1471-2180