Figure 1

Monthly incidence of serologically positive cattle in a bovine brucellosis outbreak from May 2009 to January 2011, Minas Gerais, Brazil. All cattle older than 24 months were monthly tested for brucellosis [Rose Bengal Plate Agglutination Test (RBPAT) as a screening test and the Standard Tube Agglutination Test (STAT) and 2-Mercaptoethanol Test (2ME) as a confirmatory test]. Positive animals were culled until three consecutive negative herd results were obtained. The herd was vaccinated with RB51 on May 2009 and on December 2009 (RB51 vaccinations are highlighted with arrows).